Molecular Breeding

, Volume 14, Issue 3, pp 275–282

Increased calcium in carrots by expression of an Arabidopsis H+/Ca2+ transporter

  • Sunghun Park
  • Chang-Kil Kim
  • Leonard M. Pike
  • Roberta H. Smith
  • Kendal D. Hirschi
Article

DOI: 10.1023/B:MOLB.0000047773.20175.ae

Cite this article as:
Park, S., Kim, CK., Pike, L.M. et al. Molecular Breeding (2004) 14: 275. doi:10.1023/B:MOLB.0000047773.20175.ae

Abstract

We demonstrate that carrots expressing the Arabidopsis H+/Ca2+ transporter CAX1 (Cation Exchanger 1) contained up to 50% more calcium (Ca) than plants transformed with control vectors. The CAX1-expressing carrots were fertile, and robust plant growth was seen in the majority of the transgenic plants. CAX1-expressing carrots were crossed to a commercial carrot variety to confirm that the increased Ca accumulation was mediated by CAX1-expression, and the increased Ca content was clearly correlated with the transgene. This study suggests that modulation of ion transporters could be an important means of increasing the Ca content of agriculturally important crops. To our knowledge, this study represents the first attempts to use biotechnology to increase the Ca content of an agriculturally important crop.

ArabidopsisCalciumCarrotH+/Ca2+ transporterNutrition

Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • Sunghun Park
    • 1
  • Chang-Kil Kim
    • 2
  • Leonard M. Pike
    • 1
  • Roberta H. Smith
    • 1
  • Kendal D. Hirschi
    • 3
    • 1
  1. 1.Vegetable and Fruit Improvement CenterTexas A&M University, College Station
  2. 2.Dept. of HorticultureSang-Ju National UniversityKyungpookKorea
  3. 3.Baylor College of Medicine, Depts of Pediatrics, and Human and Molecular GeneticsUSDA/ARS Children's Nutrition Research CenterHouston