Methods in Cell Science

, Volume 25, Issue 1, pp 7–14

Neural cell fate analysis in zebrafish using olig2 BAC transgenics

Authors

  • Jimann Shin
    • Department of Biological SciencesVanderbilt University
  • Hae-Chul Park
    • Department of Biological SciencesVanderbilt University
  • Jolanta M. Topczewska
    • Department of Biological SciencesVanderbilt University
  • David J. Mawdsley
    • Department of Biological SciencesVanderbilt University
  • Bruce Appel
    • Department of Biological SciencesVanderbilt University
Article

DOI: 10.1023/B:MICS.0000006847.09037.3a

Cite this article as:
Shin, J., Park, H., Topczewska, J.M. et al. Methods Cell Sci (2003) 25: 7. doi:10.1023/B:MICS.0000006847.09037.3a

Abstract

We describe here simple methods for producing transgenic zebrafish reporter lines using BAC clones. The use of BAC clones facilitates creation of useful transgenics as the large amounts of genomic DNA they contain increase the likelihood that reporter gene expression will be properly regulated. Combined with recent advances in live embryo image analysis, this strategy has the potential to greatly advance the investigation of neural cell behavior during development.

cerebellumEGFPmotor neuronolig2oligodendrocytePurkinje neuronspinal cordtransgeniczebrafish

Copyright information

© Kluwer Academic Publishers 2003