Molecular and Cellular Biochemistry

, Volume 263, Issue 1, pp 73–80

Reduced cross-bridge dependent stiffness of skinned myocardium from mice lacking cardiac myosin binding protein-C

Authors

  • Bradley M. Palmer
    • Department of Molecular Physiology and BiophysicsUniversity of Vermont
  • Bradley K. McConnell
    • Department of GeneticsHoward Hughes Medical Institute and Harvard Medical School
  • Guo Hua Li
    • Department of GeneticsHoward Hughes Medical Institute and Harvard Medical School
  • Christine E. Seidman
    • Department of Molecular Physiology and BiophysicsUniversity of Vermont
  • J.G. Seidman
    • Department of Molecular Physiology and BiophysicsUniversity of Vermont
  • Thomas C. Irving
    • Center for Synchrotron Radiation Research and Instrumentation and Department of Biological, Chemical, and Physical SciencesIllinois Institute of Technology
  • Norman R. Alpert
    • Department of Molecular Physiology and BiophysicsUniversity of Vermont
  • David W. Maughan
    • Department of Molecular Physiology and BiophysicsUniversity of Vermont
Article

DOI: 10.1023/B:MCBI.0000041849.60591.45

Cite this article as:
Palmer, B.M., McConnell, B.K., Li, G.H. et al. Mol Cell Biochem (2004) 263: 73. doi:10.1023/B:MCBI.0000041849.60591.45

Abstract

The role of cardiac myosin binding protein-C (MyBP-C) on myocardial stiffness was examined in skinned papillary muscles of wild-type (WT+/+) and homozygous truncated cardiac MyBP-C (MyBP-Ct/t) male mice. No MyBP-C was detected by gel electrophoresis or by Western blots in the MyBP-Ct/t myocardium. Rigor-bridge dependent myofilament stiffness, i.e., rigor minus relaxed stiffness, in the MyBP-Ct/t myocardium (281 ± 44 kN/m2) was 44% that in WT+/+ (633 ± 141 kN/m2). The center-to-center spacing between thick filaments as determined by X-ray diffraction in MyBP-Ct/t (45.0 ± 1.2 nm) was not significantly different from that in WT+/+ (43.2 ± 0.9 nm). The fraction of cross-sectional area comprised of myofibrils, as determined by electron microscopy, was reduced in the MyBP-Ct/t (39.9%) by 10% compared to WT+/+ (44.5%). These data suggest that the 56% reduction in rigor-bridge dependent stiffness of the skinned MyBP-Ct/t myocardium could not be due solely to a 10% reduction in the number of thick filaments per cross-sectional area and must also be due to approximately 50% reduction in the stiffness of the rigor-bridge attached thick filaments lacking MyBP-C. (Mol Cell Biochem 263: 73–80, 2004)

C-proteinisometric tensioncompliancehomozygoustruncated protein
Download to read the full article text

Copyright information

© Kluwer Academic Publishers 2004