Molecular and Cellular Biochemistry

, Volume 259, Issue 1, pp 115–129

Liver fatty acid binding protein expression enhances branched-chain fatty acid metabolism

Authors

  • Barbara P. Atshaves
    • Department of Physiology and PharmacologyTexas A&M University, TVMC, College Station
  • Stephen M. Storey
    • Department of Physiology and PharmacologyTexas A&M University, TVMC, College Station
  • Huan Huang
    • Department of Physiology and PharmacologyTexas A&M University, TVMC, College Station
  • Friedhelm Schroeder
    • Department of Physiology and PharmacologyTexas A&M University, TVMC, College Station
Article

DOI: 10.1023/B:MCBI.0000021357.97765.f2

Cite this article as:
Atshaves, B.P., Storey, S.M., Huang, H. et al. Mol Cell Biochem (2004) 259: 115. doi:10.1023/B:MCBI.0000021357.97765.f2

Abstract

Although liver fatty acid binding protein (L-FABP) is known to enhance uptake and esterification of straight-chain fatty acids such as palmitic acid and oleic acid, its effects on oxidation and further metabolism of branched-chain fatty acids such as phytanic acid are not completely understood. The present data demonstrate for the first time that expression of L-FABP enhanced initial rate and average maximal oxidation of [2,3−3H] phytanic acid 3.5− and 1.5−fold, respectively. This enhancement was not due to increased [2,3−3H] phytanic acid uptake, which was only slightly stimulated (20%) in L-FABP expressing cells after 30 min. Similarly, L-FABP also enhanced the average maximal oxidation of [9,10−3H] palmitic acid 2.2−fold after incubation for 30 min. However, the stimulation of L-FABP on palmitic acid oxidation nearly paralleled its 3.3−fold enhancement of uptake. To determine effects of metabolism on fatty acid uptake, a non-metabolizable fluorescent saturated fatty acid, BODIPY-C16, was examined by laser scanning confocal microscopy (LSCM). L-FABP expression enhanced uptake of BODIPY-C16 1.7−fold demonstrating that L-FABP enhanced saturated fatty acid uptake independent of metabolism. Finally, L-FABP expression did not significantly alter [2,3−3H] phytanic acid esterification, but increased [9,10−3H] palmitic acid esterification 4.5−fold, primarily into phospholipids (3.7−fold) and neutral lipids (9−fold). In summary, L-FABP expression enhanced branched-chain phytanic acid oxidation much more than either its uptake or esterification. These data demonstrate a potential role for L-FABP in the peroxisomal oxidation of branched-chain fatty acids in intact cells.

L-FABPfatty acidsphytanic acidpalmitic acidfatty acid oxidationfatty acid uptake

Copyright information

© Kluwer Academic Publishers 2004