Molecular and Cellular Biochemistry

, Volume 258, Issue 1, pp 65–71

Molecular characterization of a local sulfonylurea system in human adipose tissue

Authors

  • Britt G. Gabrielsson
    • Research Centre for Endocrinology and Metabolism, Endocrine DivisionSahlgrenska University Hospital
  • A. Cecilia Karlsson
    • Research Centre for Endocrinology and Metabolism, Endocrine DivisionSahlgrenska University Hospital
  • Malin Lönn
    • Research Centre for Endocrinology and Metabolism, Endocrine DivisionSahlgrenska University Hospital
  • Louise E. Olofsson
    • Research Centre for Endocrinology and Metabolism, Endocrine DivisionSahlgrenska University Hospital
  • Jenny M. Johansson
    • Research Centre for Endocrinology and Metabolism, Endocrine DivisionSahlgrenska University Hospital
  • Jarl S. Torgerson
    • Division of Body Composition and MetabolismThe Sahlgrenska Academy at Göteborg University
  • Lars Sjöström
    • Division of Body Composition and MetabolismThe Sahlgrenska Academy at Göteborg University
  • Björn Carlsson
    • Research Centre for Endocrinology and Metabolism, Endocrine DivisionSahlgrenska University Hospital
  • Staffan Edén
    • Department of Pharmacology and PhysiologyThe Sahlgrenska Academy at Göteborg University
  • Lena M.S. Carlsson
    • Research Centre for Endocrinology and Metabolism, Endocrine DivisionSahlgrenska University Hospital
Article

DOI: 10.1023/B:MCBI.0000012837.11847.c8

Cite this article as:
Gabrielsson, B.G., Karlsson, A.C., Lönn, M. et al. Mol Cell Biochem (2004) 258: 65. doi:10.1023/B:MCBI.0000012837.11847.c8

Abstract

ATP-sensitive potassium (KATP) channels are present in many cell types and link cellular metabolism to the membrane potential. These channels are heterooctamers composed of two subunits. The sulfonylurea receptor (SUR) subunits are targets for drugs that are inhibitors or openers of the KATP channels, while the inwardly rectifying K+ (Kir) subunits form the ion channel. Two different SUR genes (SUR1 and SUR2) and two different Kir6.x genes (Kir6.1 and Kir6.2) have been identified. In addition, isoforms of SUR2, SUR2A and SUR2B, have been described. We have previously performed expression profiling on pooled human adipose tissue and found high expression of SUR2. Others have reported expression of SUR1 in human adipocytes. The aim of this study was to characterize the expression of the sulfonylurea receptor complex components in human adipose tissue.

RT-PCR analysis, verified by restriction enzyme digestions and DNA sequencing, showed that SUR2B, Kir6.1 and α-endosulfine, but not SUR1, SUR2A or Kir6.2, are expressed in human adipose tissue. Real-time RT-PCR showed that SUR2B was expressed at higher levels in subcutaneous compared with omental adipose tissue in paired biopsies obtained from seven obese men (p < 0.05). Analysis of tissue distribution showed that SUR2B expression in adipose tissue was lower than that in muscle, similar to that in heart and liver, while the expression in pancreas was lower. The effect of caloric restriction was tested in obese men (n = 10) treated with very low calorie diet for 16 weeks, followed by a gradual reintroduction of ordinary food for two weeks. Biopsies were taken at week 0, 8 and 18. There was no consistent effect of weight reduction on SUR2B or Kir6.1 expression.

We conclude that the necessary components for a local sulfonylurea system are expressed in human adipose tissue and that the sulfonylurea receptor complex in this tissue is composed of SUR2B and Kir6.1. The expression of SUR2B was higher in subcutaneous compared with omental adipose tissue and was not affected by weight loss.

We conclude that the necessary components for a local sulfonylurea system are expressed in human adipose tissue and that the sulfonylurea receptor complex in this tissue is composed of SUR2B and Kir6.1. The expression of SUR2B was higher in subcutaneous compared with omental adipose tissue and was not affected by weight loss.

sulfonylurea receptor 2humanadipose tissue depotsobesityweight reduction

Copyright information

© Kluwer Academic Publishers 2004