Molecular and Cellular Biochemistry

, Volume 256, Issue 1, pp 73–81

Organization and regulation of the cytosolic NADH metabolism in the yeast Saccharomyces cerevisiae

  • Michel Rigoulet
  • Hugo Aguilaniu
  • Nicole Avéret
  • Odile Bunoust
  • Nadine Camougrand
  • Xavier Grandier-Vazeille
  • Christer Larsson
  • Inga-Lill Pahlman
  • Stephen Manon
  • Lena Gustafsson
Article

DOI: 10.1023/B:MCBI.0000009888.79484.fd

Cite this article as:
Rigoulet, M., Aguilaniu, H., Avéret, N. et al. Mol Cell Biochem (2004) 256: 73. doi:10.1023/B:MCBI.0000009888.79484.fd

Abstract

Keeping a cytosolic redox balance is a prerequisite for living cells in order to maintain a metabolic activity and enable growth. During growth of Saccharomyces cerevisiae, an excess of NADH is generated in the cytosol. Aerobically, it has been shown that the external NADH dehydrogenase, Nde1p and Nde2p, as well as the glycerol-3-phosphate dehydrogenase shuttle, comprising the cytoplasmic glycerol-3-phosphate dehydrogenase, Gpd1p, and the mitochondrial glycerol-3-phosphate dehydrogenase, Gut2p, are the most important mechanisms for mitochondrial oxidation of cytosolic NADH. In this review we summarize the recent results showing (i) the contribution of each of the mechanisms involved in mitochondrial oxidation of the cytosolic NADH, under different physiological situations; (ii) the kinetic and structural properties of these metabolic pathways in order to channel NADH from cytosolic dehydrogenases to the inner mitochondrial membrane and (iii) the organization in supramolecular complexes and, the peculiar ensuing kinetic regulation of some of the enzymes (i.e. Gut2p inhibition by external NADH dehydrogenase activity) leading to a highly integrated functioning of enzymes having a similar physiological function. The cell physiological consequences of such an organized and regulated network are discussed.

yeastin situ mitochondriaNADH dehydrogenasesrespiratory chainchannelling and kinetic regulationsupramolecular complexes

Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • Michel Rigoulet
    • 1
  • Hugo Aguilaniu
    • 1
    • 3
  • Nicole Avéret
    • 1
  • Odile Bunoust
    • 1
  • Nadine Camougrand
    • 1
  • Xavier Grandier-Vazeille
    • 1
  • Christer Larsson
    • 3
  • Inga-Lill Pahlman
    • 2
  • Stephen Manon
    • 1
  • Lena Gustafsson
    • 2
    • 3
  1. 1.IBGC-CNRS, UMR 5095Bordeaux cedexFrance
  2. 2.Department of Cellular and Molecular Biology-MicrobiologyLundberg Laboratory, Gothenburg UniversityGothenburgSweden
  3. 3.Department of Molecular BiotechnologyLundberg Laboratory, Chalmers University of TechnologyGothenburgSweden