Journal of Muscle Research & Cell Motility

, Volume 25, Issue 2, pp 119–126

Not Just a Plasma Membrane Protein: in Cardiac Muscle Cells Alpha-II Spectrin also Shows a Close Association with Myofibrils

  • Pauline M. Bennett
  • Anthony J. Baines
  • Marie-Christine Lecomte
  • Alison M. Maggs
  • Jennifer C. Pinder
Article

DOI: 10.1023/B:JURE.0000035892.77399.51

Cite this article as:
Bennett, P.M., Baines, A.J., Lecomte, M. et al. J Muscle Res Cell Motil (2004) 25: 119. doi:10.1023/B:JURE.0000035892.77399.51

Abstract

Spectrin and its associated proteins are essential for the integrity of muscle cells and there is increasing evidence for their involvement in signalling pathways as well as having a structural function in mediating stress. Spectrin is a multigene family and it is essential to determine which isoforms are present and their location in the cell. In heart muscle, we have found that one spectrin isoform, αII-spectrin, is strongly represented and, using immunofluorescence, we show that it lies within the contractile fibres near the Z-disc as well as on the cardiomyocyte plasma membrane. Electron microscopy of immunogold-labelled cryosections reveals statistically significant clustering of gold particles near the Z-disc, within and close to the edge of myofibrils. βII-spectrin and ankyrin-R and G are both known to occupy this region. We suggest that αIIβII spectrin tetramers with ankyrin organise and/or stabilise cardiac muscle cell membrane components relative to the contractile apparatus.

Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • Pauline M. Bennett
    • 1
  • Anthony J. Baines
    • 1
    • 2
  • Marie-Christine Lecomte
    • 3
  • Alison M. Maggs
    • 1
  • Jennifer C. Pinder
    • 1
  1. 1.The Randall Centre for Molecular Mechanisms of Cell Function, New Hunt's HouseKing's College London, Guy's CampusLondonUK; Tel.:
  2. 2.Department of BiosciencesUniversity of KentCanterburyUK
  3. 3.INSERM U409Faculté de Médecine Xavier BichatParis Cedex 18France