Journal of Clinical Immunology

, Volume 24, Issue 4, pp 370–378

Interleukin-4 Cellular Gene Therapy and Osteoprotegerin Decrease Inflammation-Associated Bone Resorption in Collagen-Induced Arthritis

  • Nathalie Saidenberg-Kermanac'h
  • Natacha Bessis
  • Delphine Lemeiter
  • Marie-Christine de Vernejoul
  • Marie-Christophe Boissier
  • Martine Cohen-Solal
Article

DOI: 10.1023/B:JOCI.0000029116.12371.bf

Cite this article as:
Saidenberg-Kermanac'h, N., Bessis, N., Lemeiter, D. et al. J Clin Immunol (2004) 24: 370. doi:10.1023/B:JOCI.0000029116.12371.bf
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Abstract

To evaluate the respective action of IL-4, an anti-inflammatory cytokine, and OPG, an inhibitor of bone resorption, on the inflammatory process and the associated bone resorption in collagen-induced arthritis (CIA). After CIA induction, DBA/1 mice were treated with OPG or with IL-4 DBA/1 transfected fibroblasts or both OPG + IL-4. CIA significantly improved in IL-4 groups. OPG had no effect on arthritis clinical scores but histologic scores were reduced in OPG, IL-4, and OPG + IL-4 groups vs. nontreated CIA mice. OPG increased significantly BMD and decreased by 45% D-pyridinolin levels. Moreover association of IL-4 and OPG exerted an additive effect of BMD and resorption marker (−68%). Production of IFN-γ in the supernatants of spleen cells was reduced in IL-4 treated mice. OPG had a moderate effect on IFN-γ, but potentiated the inhibitory effect of IL-4. OPG and IL-4 prevent bone loss in CIA-mice model and could have additive effects on IFN-γ secretion.

Rheumatoid arthritiscollagen-induced arthritisbone lossosteoprotegerinIL-4

Copyright information

© Plenum Publishing Corporation 2004

Authors and Affiliations

  • Nathalie Saidenberg-Kermanac'h
    • 1
  • Natacha Bessis
    • 1
  • Delphine Lemeiter
    • 1
  • Marie-Christine de Vernejoul
    • 2
  • Marie-Christophe Boissier
    • 1
  • Martine Cohen-Solal
    • 2
  1. 1.UPRES EA-3408 and Department of Rheumatology, Avicenne Hospital (AP-HP)Bobigny Medical SchoolParis 13 UniversityFrance
  2. 2.INSERM 349Lariboisière Teaching Hospital (AP-HP)ParisFrance