Article

Journal of Clinical Immunology

, Volume 24, Issue 1, pp 74-85

Increased Expression of CCL20 in Human Inflammatory Bowel Disease

  • Arthur KaserAffiliated withDivision of Gastroenterology and Hepatology, University Hospital Innsbruck
  • , Othmar LudwiczekAffiliated withDivision of Gastroenterology and Hepatology, University Hospital Innsbruck
  • , Sandra HolzmannAffiliated withDepartment of Dermatology, University Hospital Innsbruck
  • , Alexander R. MoschenAffiliated withDivision of Gastroenterology and Hepatology, University Hospital Innsbruck
  • , Günter WeissAffiliated withDivision of General Internal Medicine, Department of Medicine, University Hospital Innsbruck
  • , Barbara EnrichAffiliated withDivision of General Internal Medicine, Department of Medicine, University Hospital Innsbruck
  • , Ivo GraziadeiAffiliated withDivision of Gastroenterology and Hepatology, University Hospital Innsbruck
  • , Stefan DunzendorferAffiliated withDivision of General Internal Medicine, Department of Medicine, University Hospital Innsbruck
  • , Christian J. WiedermannAffiliated withDivision of General Internal Medicine, Department of Medicine, University Hospital Innsbruck
    • , Elisabeth MürzlAffiliated withDivision of Gastroenterology and Hepatology, University Hospital Innsbruck
    • , Eveline GraslAffiliated withDivision of Gastroenterology and Hepatology, University Hospital Innsbruck
    • , Zerina JasarevicAffiliated withDivision of Gastroenterology and Hepatology, University Hospital Innsbruck
    • , Nikolaus RomaniAffiliated withDepartment of Dermatology, University Hospital Innsbruck
    • , Felix A. OffnerAffiliated withDivision of Gastroenterology and Hepatology, University Hospital Innsbruck
    • , Herbert TilgAffiliated withDivision of Gastroenterology and Hepatology, University Hospital Innsbruck Email author 

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Abstract

Inflammatory bowel disease (IBD) constituting Crohn's disease (CD) and ulcerative colitis (UC) is related to a dysregulated T cell response. CCL20 attracts memory T lymphocytes and dendritic cells. We asked whether CCL20 expression is altered in IBD. Colonic biopsies were obtained from 114 subjects with IBD, non-IBD colitis, irritable bowel syndrome, and healthy controls. CCL20 and CCR6 mRNA expression was measured by Taqman-PCR, and protein secretion from colonic explant cultures (CEC) and its regulation by TNF-α by ELISA. CCL20, CCR6, and Langerin were identified by immunohistochemistry and immunofluorescence. CCL20 mRNA and protein were severalfold increased in involved CD and UC but not in non-IBD colitis. TNF-α increased and anti-TNF-α decreased CCL20 release in healthy control CEC but not in involved IBD colonic specimens. CCL20 localized to follicle-associated epithelium, and CCR6 to the adjacent mantle zone of lymphoid follicles. Furthermore, abundant numbers of Langerin+ immature dendritic cells were identified in the subepithelial space of IBD specimens. CCL20 might regulate the attraction of T lymphocytes and dendritic cells in IBD.

IBD chemokines lymphocytes dendritic cells epithelium