Journal of Clinical Immunology

, Volume 24, Issue 1, pp 74–85

Increased Expression of CCL20 in Human Inflammatory Bowel Disease

Authors

  • Arthur Kaser
    • Division of Gastroenterology and HepatologyUniversity Hospital Innsbruck
  • Othmar Ludwiczek
    • Division of Gastroenterology and HepatologyUniversity Hospital Innsbruck
  • Sandra Holzmann
    • Department of DermatologyUniversity Hospital Innsbruck
  • Alexander R. Moschen
    • Division of Gastroenterology and HepatologyUniversity Hospital Innsbruck
  • Günter Weiss
    • Division of General Internal Medicine, Department of MedicineUniversity Hospital Innsbruck
  • Barbara Enrich
    • Division of General Internal Medicine, Department of MedicineUniversity Hospital Innsbruck
  • Ivo Graziadei
    • Division of Gastroenterology and HepatologyUniversity Hospital Innsbruck
  • Stefan Dunzendorfer
    • Division of General Internal Medicine, Department of MedicineUniversity Hospital Innsbruck
  • Christian J. Wiedermann
    • Division of General Internal Medicine, Department of MedicineUniversity Hospital Innsbruck
  • Elisabeth Mürzl
    • Division of Gastroenterology and HepatologyUniversity Hospital Innsbruck
  • Eveline Grasl
    • Division of Gastroenterology and HepatologyUniversity Hospital Innsbruck
  • Zerina Jasarevic
    • Division of Gastroenterology and HepatologyUniversity Hospital Innsbruck
  • Nikolaus Romani
    • Department of DermatologyUniversity Hospital Innsbruck
  • Felix A. Offner
    • Division of Gastroenterology and HepatologyUniversity Hospital Innsbruck
    • Division of Gastroenterology and HepatologyUniversity Hospital Innsbruck
Article

DOI: 10.1023/B:JOCI.0000018066.46279.6b

Cite this article as:
Kaser, A., Ludwiczek, O., Holzmann, S. et al. J Clin Immunol (2004) 24: 74. doi:10.1023/B:JOCI.0000018066.46279.6b

Abstract

Inflammatory bowel disease (IBD) constituting Crohn's disease (CD) and ulcerative colitis (UC) is related to a dysregulated T cell response. CCL20 attracts memory T lymphocytes and dendritic cells. We asked whether CCL20 expression is altered in IBD. Colonic biopsies were obtained from 114 subjects with IBD, non-IBD colitis, irritable bowel syndrome, and healthy controls. CCL20 and CCR6 mRNA expression was measured by Taqman-PCR, and protein secretion from colonic explant cultures (CEC) and its regulation by TNF-α by ELISA. CCL20, CCR6, and Langerin were identified by immunohistochemistry and immunofluorescence. CCL20 mRNA and protein were severalfold increased in involved CD and UC but not in non-IBD colitis. TNF-α increased and anti-TNF-α decreased CCL20 release in healthy control CEC but not in involved IBD colonic specimens. CCL20 localized to follicle-associated epithelium, and CCR6 to the adjacent mantle zone of lymphoid follicles. Furthermore, abundant numbers of Langerin+ immature dendritic cells were identified in the subepithelial space of IBD specimens. CCL20 might regulate the attraction of T lymphocytes and dendritic cells in IBD.

IBDchemokineslymphocytesdendritic cellsepithelium

Copyright information

© Plenum Publishing Corporation 2004