Journal of Bioenergetics and Biomembranes

, Volume 36, Issue 4, pp 317–321

Astrocyte Mitochondria in In Vitro Models of Ischemia

  • Laura L. Dugan
  • Jeong-Sook Kim-Han
Article

DOI: 10.1023/B:JOBB.0000041761.61554.44

Cite this article as:
Dugan, L.L. & Kim-Han, JS. J Bioenerg Biomembr (2004) 36: 317. doi:10.1023/B:JOBB.0000041761.61554.44

Abstract

There is growing evidence that preservation of mitochondrial respiratory function during cerebral ischemia–reperfusion predicts the ultimate extent of tissue injury. Because neurons are selectively vulnerable to ischemic injury, many studies have focused on neuronal mitochondrial dysfunction in ischemia. However, positron emission tomography (PET) studies in animals and humans suggest that non-neuronal cells such as astrocytes may also experience mitochondrial metabolic compromise that contributes to ischemic necrosis. Astrocytes carry out a number of functions that are critical to normal nervous system function, including uptake of neurotransmitters, regulation of pH and ion concentrations, and metabolic support of neurons. Mitochondria are important for many of these actions. We have used a cell culture model of stroke, oxygen–glucose deprivation (OGD), to study the response of astrocyte mitochondria to ischemia, and to evaluate how changes in astrocyte mitochondrial function might affect neuronal survival and recovery after ischemia.

Tetramethylrhodamine ethyl ester cytochrome c mitochondrial permeability transition pore nitric oxide synthase cyclosporin A confocal microscopy cortical cell cultures brain slice 

Copyright information

© Springer Science+Business Media, Inc. 2004

Authors and Affiliations

  • Laura L. Dugan
    • 1
  • Jeong-Sook Kim-Han
    • 1
  1. 1.Department of NeurologyWashington UniversitySt. LouisMissouri

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