Journal of Applied Phycology

, Volume 16, Issue 4, pp 291–296

Local and chemical distribution of phlorotannins in brown algae

  • Toshiyuki Shibata
  • Shigeo Kawaguchi
  • Yoichiro Hama
  • Masanori Inagaki
  • Kuniko Yamaguchi
  • Takashi Nakamura
Article

DOI: 10.1023/B:JAPH.0000047781.24993.0a

Cite this article as:
Shibata, T., Kawaguchi, S., Hama, Y. et al. Journal of Applied Phycology (2004) 16: 291. doi:10.1023/B:JAPH.0000047781.24993.0a

Abstract

The local and chemical distribution of phlorotannins among the Japanese Laminariaceae, Eisenia bicyclis, Ecklonia cava and Ecklonia kurome, was investigated. As a result of light microscopy observations with vanillin-HCl staining, phlorotannins were found to be accumulated within the vegetative cells of the outer cortical layer of the thalli, regardless of the species, stage of growth or organ. Crude phlorotannins comprised about 3.0% of the algal powder for each of the algae. High-performance liquid chromatography (HPLC) showed that the phlorotannins of E. bicyclis were composed of phloroglucinol (0.9%), phloroglucinol tetramer (4.4%), eckol (7.5%), phlorofucofuroeckol A (21.9%), dieckol (23.4%), and 8,8'-bieckol (24.6%), plus some other unknown phenolic compounds (17.3%). The composition of the phlorotannins differed little among the Laminariaceae, except for a significantly larger amount of the tetramer, MW 478, in E. bicyclis.

brown algaEcklonia cavaEcklonia kuromeEisenia bicyclisHPLClight microscopyphlorotanninTLCvanillin-HCl

Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • Toshiyuki Shibata
    • 1
  • Shigeo Kawaguchi
    • 2
  • Yoichiro Hama
    • 3
  • Masanori Inagaki
    • 4
  • Kuniko Yamaguchi
    • 2
  • Takashi Nakamura
    • 2
  1. 1.Venture Business LaboratorySaga UniversitySagaJapan.
  2. 2.Laboratory of Marine Biology, Graduate School of AgricultureKyushu UniversityFukuokaJapan
  3. 3.Department of Applied Biological Science, Faculty of AgricultureSaga UniversitySagaJapan
  4. 4.Laboratory of Natural Product Chemistry, Graduate School of Pharmaceutical SciencesKyushu UniversityFukuokaJapan