Background Subtraction in Experimental Data Arrays Illustrated by the Example of Raman Spectra and Fluorescent Gel Electrophoresis Patterns
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- Mikhailyuk, I.K. & Razzhivin, A.P. Instruments and Experimental Techniques (2003) 46: 765. doi:10.1023/B:INET.0000008930.87741.b9
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A method for background signal subtraction from one-dimensional experimental data arrays (optical spectra, chromatograms) and two-dimensional arrays (electrophoregrams) is based on the difference in the geometric properties (radii of curvature) of the curves of the useful and background signals. To demonstrate the efficiency of this method, it is applied to the Raman spectrum of chymotrypsin and a fluorescent gel electrophoregram of hepatitis B DNA fragments stained with ethydium bromide.