Digestive Diseases and Sciences

, Volume 49, Issue 1, pp 122–132

Indigestible Disaccharides Open Tight Junctions and Enhance Net Calcium, Magnesium, and Zinc Absorption in Isolated Rat Small and Large Intestinal Epithelium

  • Hitoshi Mineo
  • Midori Amano
  • Hideyuki Chiji
  • Norihiro Shigematsu
  • Fusao Tomita
  • Hiroshi Hara
Article

DOI: 10.1023/B:DDAS.0000011613.11671.c7

Cite this article as:
Mineo, H., Amano, M., Chiji, H. et al. Dig Dis Sci (2004) 49: 122. doi:10.1023/B:DDAS.0000011613.11671.c7

Abstract

The effects of three indigestible disaccharides on net calcium (Ca), magnesium (Mg), and zinc (Zn) transport in isolated rat jejunal, ileal, cecal, and colonic epithelium were determined. Permeability of fluorescein isothiocynate–dextran-4 (FD4) and transepithelial electrical resistance (TEER), which vary according to tight junction (TJ) activity in the intestinal mucosa, were also determined. The addition of 1–100 mM melibiose, difructose anhydride (DFA) III, or DFA IV to the mucosal medium increased the net absorption of the three minerals and FD4 permeability, while decreasing TEER dose dependently in the four intestinal portions. Positive linear relations were found between the net transport of the three minerals and FD4 passage in all portions of the intestine, whereas negative linear relations were observed between net absorption of the three minerals and TEER. We concluded that the three indigestible saccharides directly affect the epithelial tissue and open TJs, thereby promoting Ca, Mg, and Zn absorption in the small and large intestine in vitro.

mineral absorption indigestible saccharide Ussing chamber intestine tight junction rat 

Copyright information

© Plenum Publishing Corporation 2004

Authors and Affiliations

  • Hitoshi Mineo
    • 1
    • 2
  • Midori Amano
    • 1
    • 3
  • Hideyuki Chiji
    • 3
  • Norihiro Shigematsu
    • 4
  • Fusao Tomita
    • 2
  • Hiroshi Hara
    • 2
  1. 1.Northern Advancement Center for Science and TechnologyColabo-Hokkaido, Sapporo
  2. 2.Division of Applied Bioscience, Graduate School of AgricultureHokkaido UniversitySapporoJapan
  3. 3.Department of Food Science and Human Nutrition, Faculty of Human Life ScienceFuji Women's UniversityIshikari
  4. 4.Central Research CenterFancl CorporationYokohama