Chromosome Research

, 12:417

Construction of novel Brassica napus genotypes through chromosomal substitution and elimination using interploid species hybridization

Article

DOI: 10.1023/B:CHRO.0000034722.66981.94

Cite this article as:
Li, M., Qian, W., Meng, J. et al. Chromosome Res (2004) 12: 417. doi:10.1023/B:CHRO.0000034722.66981.94

Abstract

A synthetic Brassica napus rapeseed with genome composition of ArArCcCc, made by combining Ar from B. rapa (ArAr) and Cc from B. carinata (BcBcCcCc), is valuable for making new genes available to breeders and gaining heterosis in crosses. An intergenomic hybrid AnArCnCc was made from a hybrid between natural Brassica napus (AnAnCnCn) and a synthetic rapeseed. To construct the synthetic Brassica napus, hexaploid plants (2n=54, ArArBcBcCcCc) were first obtained through chromosome doubling of trigenomic hybrids (2n=27, ArBcCc) between Brassica carinata (2n=34) and B. rapa (2n=20). Pentaploid hybrids (2n=46, ArAnBcCcCn) were then produced by crossing the hexaploid with the pollen of natural B. napus (2n=38). Chromosomes with dual and single Bc genomes were observed in somatic cells of hexaploid and pentaploid plants. About 80% of pollen mother cells of pentaploid hybrids had 19 or more bivalents, indicating that the bivalents from Ar/An and Cc/Cn chromosomes were normally formed. The occurrence of trivalents and quadrivalents at diakinesis suggested that Bc, An and Ar or Bc, Cn and Cc homologous pairing and exchange might happen. The variable number of laggards, 3 and 4 in most cases, were observed in the majority of PMCs at anaphase. Results from genomic in situ hybridization showed that the laggards belonged mainly to the Bc genome, suggesting that the Bc genome could be eliminated in the gametesof pentaploid hybrids. 16.15% of seeds derived from self-pollinated pentaploids have 38 chromosomes, and 90% of 38-chromosome seeds were completely excluded Bc genome. The cytological results of this experiment suggested that it is possible to obtain new materials with genome composition of ArArCcCc for rapeseed breeding.

Brassicagenomicsin situ hybridizationpentaploid

Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • Maoteng Li
    • 1
  • Wei Qian
    • 1
  • Jinling Meng
    • 1
  • Zongyun Li
    • 3
  1. 1.National Key Lab of Crop ImprovementHuazhong Agricultural UniversityWuhanP.R. China
  2. 2.School of Life Science & TechnologyHuazhong University of Science & TechnologyWuhanP.R. China
  3. 3.Department of BiologyXuzhou Normal UniversityXuzhouP.R. China