Chromosome Research

, Volume 12, Issue 4, pp 337–343

Generation of Chromosome Painting Probes from Single Chromosomes by Laser Microdissection and Linker-Adaptor PCR

Authors

  • Stefan Thalhammer
    • Department für GeowissenschaftenUniversity of Munich
  • Sabine Langer
    • Institut für HumangenetikTechnische Universität München
    • Institut für HumangenetikGSF-Forschungszentrum für Umwelt und Gesundheit
  • Michael R. Speicher
    • Institut für HumangenetikTechnische Universität München
    • Institut für HumangenetikGSF-Forschungszentrum für Umwelt und Gesundheit
  • Wolfgang M. Heckl
    • Department für GeowissenschaftenUniversity of Munich
  • Jochen B. Geigl
    • Institut für HumangenetikTechnische Universität München
    • Institut für HumangenetikGSF-Forschungszentrum für Umwelt und Gesundheit
Article

DOI: 10.1023/B:CHRO.0000034132.77192.5f

Cite this article as:
Thalhammer, S., Langer, S., Speicher, M.R. et al. Chromosome Res (2004) 12: 337. doi:10.1023/B:CHRO.0000034132.77192.5f

Abstract

Fluorescence in situ hybridization (FISH) plays an essential role in research and clinical diagnostics. The versatility and resolution of FISH depends critically on the probe set used. Here, we describe an improved approach for the generation of specific DNA probes from single copies of chromosomes. Single chromosomes or single chromosomal regions were microdissected by laser pressure catapulting and amplified using linker-adaptor PCR. The probes were labeled and tested in various scenarios including multicolor-FISH experiments employing up to seven different fluorochromes. FISH confirmed the specific and even staining of the respective chromosomal regions. Furthermore, the capability of these probes to detect even small translocations (<3 Mb) suggests that the dissected regions are completely represented in the generated painting probes.

chromosome paintinglaser microdissectionlinker-adaptor PCRmolecular cytogenetics

Copyright information

© Kluwer Academic Publishers 2004