Endogenous Dopamine Maintains Synchronous Oscillation of Intracellular Calcium in Primary Cultured-Mouse Midbrain Neurons
Purchase on Springer.com
$39.95 / €34.95 / £29.95*
Rent the article at a discountRent now
* Final gross prices may vary according to local VAT.
We demonstrated synchronous oscillation of intracellular Ca2+ in cultured-mouse midbrain neurons. This synchronous oscillation was thought to result from spontaneous and synchronous neural bursts in a synaptic neural network. We also examined the role of endogenous dopamine in neural networks showing synchronous oscillation. Immunocytochemical study revealed a few tyrosine hydroxylase (TH)-positive dopaminergic neurons, and that cultured neurons expressed synaptophysin and synapsin I. Western blot analyses comfirmed synaptophysin, TH, and 2 types of dopamine receptor (DR), D1R and D2R expression. The synchronous oscillation in midbrain neurons was abolished by the application of R(−)-2-amino-5-phosphonopentanoic acid (AP-5) as an N-methyl-D-aspartate receptor (NMDAR) antagonist. This result suggests that the synchronous oscillation in midbrain neurons requires glutamatergic transmissions, as was the case in previously reported cortical neurons. SCH-12679, a D1R antagonist, inhibited synchronous oscillation in midbrain neurons, while raclopride, a D2R antagonist, induced a transient increase of intracellular Ca2+ and inhibited synchronous oscillation. We consider that endogenous dopamine maintains synchronous oscillation of intracellular Ca2+ through D1R and D2R, and that these DRs regulate intracellular Ca2+in distinctly different ways. Synchronous oscillation of midbrain neurons would be a useful tool for in vitro researches into various neural disorders directly or indirectly caused by dopaminergic neurons.
- Basma, A. N., Morris, E. J., Nicklas, W. J., and Geller, H. M. (1995). L-dopa cytotoxicity to PC12 cells in culture is via its autoxidation. J. Neurochem. 64:825-832.
- Garaschuk, O., Hanse, E., and Konnerth, A. (1998). Developmental profile and synaptic origin of early network oscillations in the CA1 region of rat neonatal hippocampus. J. Physiol. (Lond.) 507:219-236.
- Ghosh, A., and Greenberg, M. E. (1995). Calcium signaling in neurons: Molecular mechanisms and cellular consequences. Science 268:239-247.
- Gray, C. M., Konig, P., Engel, A. K., and Singer, W. (1989). Oscillatory responses in cat visual cortex exhibit inter-columnar synchronization which reflects global stimulus properties. Nature 338:334-337.
- Heyer, E. J. (1986). Dopamine antagonists reduce spontaneous electrical activity in cultured mammalian neurons from ventral mesencephalon. Brain Res. 382:404-408.
- Ichikawa, M., Muramoto, K., Kobayashi, K., Kawahara, M., and Kuroda, Y. (1993). Formation and maturation of synapses in primary cultures of rat cerebral cortical cells: An electron microscopic study. Neurosci. Res. Suppl. 16:95-103.
- Kim, K. M., Nakajima, Y., and Nakajima, S. (1995). G protein-coupled inward rectifier modulated by dopamine agonists in cultured substantia nigra neurons. Neuroscience 69:1145-1158.
- Kimura, N., Nakamura, S. I., Honda, T., Takashima, A., Nakayama, H., Ono, F., Sakakibara, I., Doi, K., Kawamura, S., and Yoshikawa, Y. (2001). Age-related changes in the localization of presenilin-1 in cynomolgus monkey brain. Brain Res. 922:30-41.
- Kuroda, Y., Ichikawa, M., Muramoto, K., Kobayashi, K., Matsuda, Y., Ogura, A., and Kudo, Y. (1992). Block of synapse formation between cerebral cortical neurons by a protein kinase inhibitor. Neurosci. Lett. 135:255-258.
- Langston, J. W. (1996). The etiology of Parkinson's disease with emphasis on the MPTP story. Neurology 47:S153-S160.
- Maurice, N., Tkatch, T., Meisler, M., Sprunger, L. K., and Surmeier, D. J. (2001). D1/D5 dopamine receptor activation differentially modulates rapidly inactivating and persistent sodium currents in prefrontal cortex pyramidal neurons. J. Neurosci. 21:2268-2277.
- Mena, M. A., Davila, V., and Sulzer, D. (1997a). Neurotrophic effects of L-dopa in postnatal midbrain dopamine neuron/cortical astrocyte cocultures. J. Neurochem. 69:1398-1408.
- Mena, M. A.,Khan, U., Togasaki, D. M., Sulzer, D., Epstein, C. J., and Przedborski, S. (1997b). Effects of wild-type and mutated copper/zinc superoxide dismutase on neuronal survival and L-dopa-induced toxicity in postnatal midbrain culture. J. Neurochem. 69:21-33.
- Nakamura, K., Mikami, A., and Kubota, K. (1992). Oscillatory neuronal activity related to visual short-term memory in monkey temporal pole. Neuroreport 3:117-120.
- Ng, G. Y., Trogadis, J., Stevens, J., Bouvier, M., O'Dowd, B. F., and George, S. R. (1995). Agonist-induced desensitization of dopamine D1 receptor-stimulated adenylyl cyclase activity is temporally and biochemically separated from D1 receptor internalization. Proc. Natl. Acad. Sci. U.S.A. 92:10157-10161.
- Ogura, A., Iijima, T., Amano, T., and Kudo, Y. (1987). Optical monitoring of excitatory synaptic activity between cultured hippocampal neurons by a multi-site Ca2+ fluorometry. Neurosci. Lett. 78:69-74.
- Ralph-Williams, R. J., Lehmann-Masten, V., Otero-Corchon, V., Low, M. J., and Geyer, M. A. (2002). Differential effects of direct and indirect dopamine agonists on prepulse inhibition: A study in D1 and D2 receptor knock-out mice. J. Neurosci. 22:9604-9611.
- Robinson, H. P., Kawahara, M., Jimbo, Y., Torimitsu, K., Kuroda, Y., and Kawana, A. (1993). Periodic synchronized bursting and intracellular calcium transients elicited by low magnesium in cultured cortical neurons. J. Neurophysiol. 70:1606-1616.
- Senogles, S. E. (1994). The D2 dopamine receptor isoforms signal through distinct Gi alpha proteins to inhibit adenylyl cyclase. A study with site-directed mutant Gi alpha proteins. J. Biol. Chem. 269:23120-23127.
- Shaner, A. (1999). Delusions, superstitious conditioning and chaotic dopamine neurodynamics. Med. Hypotheses 52:119-123.
- Suzuki, H., Shishido, T., Watanabe, Y., Abe, H., Shiragata, M., Honda, K., Horikoshi, R., and Niwa, S. (1997). Changes of behavior and monoamine metabolites in the rat brain after repeated methamphetamine administration: Effects of duration of repeated administration. Prog. Neuropsychopharmacol. Biol. Psychiatry 21:359-369.
- Svensson, E., Grillner, S., and Parker, D. (2001). Gating and braking of short-and long-term modulatory effects by interactions between colocalized neuromodulators. J. Neurosci. 21:5984-5992.
- Vallar, L., and Meldolesi, J. (1989). Mechanisms of signal transduction at the dopamine D2 receptor. Trends Pharmacol. Sci. 10:74-77.
- van den Pol, A. N., Cao, V., and Belousov, A. B. (1996). Dopamine enhancement and depression of glutamate-regulated calcium and electrical activity in hypothalamic neurons. J. Neurophysiol. 76:3934-3948.
- Endogenous Dopamine Maintains Synchronous Oscillation of Intracellular Calcium in Primary Cultured-Mouse Midbrain Neurons
Cellular and Molecular Neurobiology
Volume 24, Issue 1 , pp 51-61
- Cover Date
- Print ISSN
- Online ISSN
- Kluwer Academic Publishers-Plenum Publishers
- Additional Links
- dopamine receptor
- neural burst
- Industry Sectors
- Author Affiliations
- 1. Department of Biomedical Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan
- 3. Core Research for Evolutional Science and Technology, Japan Science and Technology Corporation, Saitama, Japan
- 2. Department of Molecular and Cellular Neurobiology, Tokyo Metropolitan Institute for Neuroscience, Tokyo, Japan