Cellular and Molecular Neurobiology

, Volume 24, Issue 1, pp 137–147

Role of ARF4L in Recycling Between Endosomes and the Plasma Membrane

  • Taiichi Katayama
  • Kazunori Imaizumi
  • Takunari Yoneda
  • Manabu Taniguchi
  • Akiko Honda
  • Takayuki Manabe
  • Junichi Hitomi
  • Kayoko Oono
  • Kousuke Baba
  • Shingo Miyata
  • Shinsuke Matsuzaki
  • Koichi Takatsuji
  • Masaya Tohyama
Article

DOI: 10.1023/B:CEMN.0000012719.12015.ec

Cite this article as:
Katayama, T., Imaizumi, K., Yoneda, T. et al. Cell Mol Neurobiol (2004) 24: 137. doi:10.1023/B:CEMN.0000012719.12015.ec

Abstract

The human ADP-ribosylation factor-like protein, ARF4L is a member of the ARF family, which are small GTP-binding proteins that play significant roles in vesicle transport and protein secretion. However, little is known about the physiological roles of ARF4L. In this study, to understand the biological functions of ARF4L, we carried out immunocytochemical analysis of ARF4L molecules with mutations in the functional domains. ARF4L was shown to be distributed to the plasma membrane following binding to GTP (Q80L), and into endosomes following binding to GDP (T35N). Moreover, the inactive-form of ARF4L (T35N) causes localization of transferrin receptors to the endosomal compartment, while the active form (Q80L) causes transport to the plasma membrane. These findings indicate that ARF4L drive the transport of cargo protein and subsequent fusion of recycling vesicles with the plasma membrane for maintenance of the cell surface.

ARF4L membrane trafficking GTP-binding plasma membrane endosome 

Copyright information

© Plenum Publishing Corporation 2004

Authors and Affiliations

  • Taiichi Katayama
    • 1
    • 2
  • Kazunori Imaizumi
    • 2
    • 3
  • Takunari Yoneda
    • 1
    • 2
  • Manabu Taniguchi
    • 1
    • 2
  • Akiko Honda
    • 1
    • 2
  • Takayuki Manabe
    • 1
    • 2
  • Junichi Hitomi
    • 1
    • 2
  • Kayoko Oono
    • 1
    • 2
  • Kousuke Baba
    • 1
    • 2
  • Shingo Miyata
    • 1
    • 2
  • Shinsuke Matsuzaki
    • 1
    • 2
  • Koichi Takatsuji
    • 4
  • Masaya Tohyama
    • 1
    • 2
  1. 1.Department of Anatomy & Neuroscience, Graduate School of MedicineOsaka UniversitySuita, OsakaJapan
  2. 2.Core Research for Evolutional Science and Technology (CREST)KawaguchiJapan
  3. 3.Department of Structural Cell BiologyNara Institute of Science and TechnologyIkoma, NaraJapan
  4. 4.Osaka Prefectural College of NursingHabikino, OsakaJapan

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