Cell and Tissue Banking

, Volume 4, Issue 2, pp 95–100

Properties of Air Dried Radiation Processed Amniotic Membranes under Different Storage Conditions

  • Rita Singh
  • Pallavi Gupta
  • Pawan Kumar
  • Ashok Kumar
  • M.P. Chacharkar
Article

DOI: 10.1023/B:CATB.0000007030.72031.12

Cite this article as:
Singh, R., Gupta, P., Kumar, P. et al. Cell Tissue Banking (2003) 4: 95. doi:10.1023/B:CATB.0000007030.72031.12

Abstract

Amniotic membranes collected from the placentae of screened donors were processed, air dried and sterilized by gamma irradiation at 25 kGy. Effect of storage under different temperature and humidity conditions (10°C, RH 80–90%; 10°C, RH 40–50%; 40°C, RH 50–60% and 40°C, RH 10–20%) on the properties of the membrane were examined. Infrared (IR) spectral scanning was carried out to examine degradation or change if any in the tissue under different storage conditions. The degradation of amnion on irradiation with gamma rays or during storage after irradiation would tend to produce the relative variation in IR absorption troughs. This kind of addendum was absent in all the samples indicating no qualitative change in the material property of amnion. Water absorption and water vapour transmission rate (WVTR) of the membrane remained unchanged even after 6 months. No effect on the microbial permeability of membrane was observed during storage. The amniotic membranes were found to be impermeable to different strains of bacteria – Bacillus, Escherichia coli, Pseudomonas, Citrobacter, Flavimonas and Staphylococcus. The results indicate that amniotic membranes processed by air-drying are stable and can be stored under different environmental conditions without compromise to their clinical performance.

air dried amniotic membrane infrared spectra microbial permeability properties radiation storage conditions water absorption water vapour transmission rate 

Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • Rita Singh
    • 1
  • Pallavi Gupta
    • 1
  • Pawan Kumar
    • 1
  • Ashok Kumar
    • 1
  • M.P. Chacharkar
    • 1
  1. 1.Defence LaboratoryJodhpurIndia

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