Breast Cancer Research and Treatment

, Volume 84, Issue 2, pp 99–105

Detection of LOH and Mitochondrial DNA Alterations in Ductal Lavage and Nipple Aspirate Fluids from High-risk Patients

  • Claudine Isaacs
  • Luciane R. Cavalli
  • Yoram Cohen
  • Marie Pennanen
  • Lalita K. Shankar
  • Matthew Freedman
  • Baljit Singh
  • Minetta Liu
  • Ann Gallagher
  • Janice D. Rone
  • Robert B. Dickson
  • David Sidransky
  • Bassem R. Haddad
Article

DOI: 10.1023/B:BREA.0000018406.03679.2e

Cite this article as:
Isaacs, C., Cavalli, L.R., Cohen, Y. et al. Breast Cancer Res Treat (2004) 84: 99. doi:10.1023/B:BREA.0000018406.03679.2e

Abstract

We describe a method for the isolation of free DNA from ductal lavage (DL) and nipple aspirate fluid (NAF), and its evaluation for the presence of LOH at the BRCA1 and FHIT genes and for mitochondrial DNA (mtDNA) mutations at the D310 marker, to improve early detection of breast cancer. We evaluated 26 DL and six NAF samples from 14 women of known BRCA1 status, who have no clinical evidence of breast tumors: nine mutation carriers and five non-carriers. LOH studies at the BRCA1 locus were possible in 19/26 DL samples, and at the FHIT locus in 16/26 samples. In 4/9 mutation carriers we found LOH at the BRCA1 allele, and in two of these we also found LOH at the FHIT allele. In one of the mutation carriers with BRCA1 LOH, invasive breast cancer was subsequently detected, and the tumor showed the same LOH as the DL. In one of the true negatives, BRCA1 and FHIT LOH were detected. The mitochondrial studies were possible in all 26 DL samples and a somatic mutation was found in 3/9 carriers, two of whom also had LOH at the BRCA1 locus, and in none of the non-carriers. mtDNA mutation evaluation was possible in 4/6 NAF samples. The NAF and DL results were concordant. One NAF sample from a BRCA1 patient showed a mtDNA mutation. Our data demonstrates the feasibility of performing molecular studies using the free DNA present in the ductal fluid, while the intact cells can be used for cytologic studies.

breast cancerductal lavageloss of heterozygositymitochondrial mutationsnipple aspirate fluid

Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • Claudine Isaacs
    • 1
    • 2
  • Luciane R. Cavalli
    • 1
    • 2
  • Yoram Cohen
    • 8
  • Marie Pennanen
    • 1
    • 3
  • Lalita K. Shankar
    • 1
    • 5
  • Matthew Freedman
    • 1
    • 4
  • Baljit Singh
    • 1
    • 2
    • 6
  • Minetta Liu
    • 1
    • 2
  • Ann Gallagher
    • 1
    • 2
  • Janice D. Rone
    • 1
    • 2
  • Robert B. Dickson
    • 1
    • 2
  • David Sidransky
    • 8
  • Bassem R. Haddad
    • 1
    • 2
    • 7
  1. 1.Lombardi Comprehensive Cancer CenterGeorgetown UniversityWashington, DCUSA
  2. 2.Department of OncologyGeorgetown UniversityWashington, DCUSA
  3. 3.Department of SurgeryGeorgetown UniversityWashington, DCUSA
  4. 4.Department of RadiologyGeorgetown UniversityWashington, DCUSA
  5. 5.Department of Nuclear MedicineGeorgetown UniversityWashington, DCUSA
  6. 6.Department of PathologyGeorgetown UniversityWashington, DCUSA
  7. 7.Department of Obstetrics and GynecologyGeorgetown UniversityWashington, DCUSA
  8. 8.Department of Otolaryngology-Head and Neck SurgeryThe Johns Hopkins UniversityBaltimoreUSA