Breast Cancer Research and Treatment

, Volume 82, Issue 3, pp 155–164

Regression of Cutaneous Tumor Lesions in Patients Intratumorally Injected with a Recombinant Single-chain Antibody-toxin Targeted to ErbB2/HER2

  • Marc Azemar
  • Sirus Djahansouzi
  • Elke Jäger
  • Christine Solbach
  • Mathias Schmidt
  • Alexander B. Maurer
  • Klaus Mross
  • Clemens Unger
  • Gunter von Minckwitz
  • Peter Dall
  • Bernd Groner
  • Winfried S. Wels
Article

DOI: 10.1023/B:BREA.0000004371.48757.19

Cite this article as:
Azemar, M., Djahansouzi, S., Jäger, E. et al. Breast Cancer Res Treat (2003) 82: 155. doi:10.1023/B:BREA.0000004371.48757.19

Abstract

ScFv(FRP5)-ETA is a recombinant single-chain antibody-toxin with binding specificity for ErbB2/HER2. Previously potent antitumoral activity of the molecule against ErbB2 overexpressing tumor cells was demonstrated in vitro and in animal models. Here we report on the first application of scFv(FRP5)-ETA in human cancer patients summarizing case reports collected in four different clinical centers. Eleven patients suffering from metastatic breast and colorectal cancers and from malignant melanoma were treated on a compassionate-use basis by intratumoral injection of scFv(FRP5)-ETA into cutaneous lesions once daily for 7–10 days. Total daily doses ranged from 60 to 900 µg, and total doses per treatment cycle ranged from 0.6 to 6.0 mg. Treatment caused injected tumors to shrink in six of the 10 cases evaluated (60%). Complete regression of injected tumor nodules was accomplished in four patients (40%) and partial reduction in tumor size in another two patients (20%). Adverse reactions were restricted to local symptoms such as pain and inflammation at injection sites which were fully reversible. Only in one patient treated at the highest daily doses systemic liver toxicity of grade 2 was observed and treatment was discontinued on day 7. No hematologic, renal, and/or cardiovascular toxicities were noted. Our results demonstrate that local therapy with scFv(FRP5)-ETA can be effective against ErbB2 expressing tumors justifying further clinical development of this reagent.

ErbB2 Pseudomonas exotoxin A scFv(FRP5)-ETA single chain Fv antibody 

Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • Marc Azemar
    • 1
  • Sirus Djahansouzi
    • 2
  • Elke Jäger
    • 3
  • Christine Solbach
    • 4
  • Mathias Schmidt
    • 1
  • Alexander B. Maurer
    • 6
    • 7
  • Klaus Mross
    • 1
  • Clemens Unger
    • 1
  • Gunter von Minckwitz
    • 4
  • Peter Dall
    • 2
  • Bernd Groner
    • 5
  • Winfried S. Wels
    • 5
  1. 1.Tumor Biology CenterFreiburgGermany
  2. 2.Department of Obstetrics and GynecologyHeinrich-Heine-UniversityDüsseldorfGermany
  3. 3.Hämatologie-OnkologieMedizinische Klinik IIKrankenhaus NordwestGermany
  4. 4.Department of Gynecology and Obstetrics, School of MedicineJohann Wolfgang Goethe-UniversityGermany
  5. 5.Department Oncology ResearchAltana Pharma AGKonstanzGermany
  6. 6.Chemotherapeutisches Forschungsinstitut Georg-Speyer-HausFrankfurt am MainGermany
  7. 7.G2M Cancer Drugs AGFrankfurt am MainGermany