Biomedical Microdevices

, Volume 6, Issue 2, pp 139–147

Biological Laser Printing: A Novel Technique for Creating Heterogeneous 3-dimensional Cell Patterns


  • J.A. Barron
    • Naval Research Laboratory
  • P. Wu
    • Southern Oregon University
  • H.D. Ladouceur
    • Naval Research Laboratory
    • Naval Research Laboratory

DOI: 10.1023/B:BMMD.0000031751.67267.9f

Cite this article as:
Barron, J., Wu, P., Ladouceur, H. et al. Biomedical Microdevices (2004) 6: 139. doi:10.1023/B:BMMD.0000031751.67267.9f


We have developed a laser-based printing technique, called biological laser printing (BioLP™). BioLP is a non-contact, orifice-free technique that rapidly deposits fL to nL scale volumes of biological material with spatial accuracy better than 5 μm. The printer's orifice-free nature allows for transfer of a wide range of biological material onto a variety of substrates. Control of transfer is performed via a computer-aided design/computer-aided manufacturing (CAD/CAM) system which allows for deposition rates up to 100 pixels of biological material per second using the current laser systems. In this article, we present a description of the apparatus, a model of the transfer process, and a comparison to other biological printing techniques. Further, examples of current system capabilities, such as adjacent deposition of multiple cell types, large-scale cell arrays, and preliminary experiments on creating multi-layer cell constructs are presented. These cell printing experiments not only demonstrate near 100% viability, they also are the first steps toward using BioLP to create heterogeneous 3-dimensional constructs for use in tissue engineering applications.

biological laser printing (BioLP)cell printingcell patterningtissue engineering

Copyright information

© Kluwer Academic Publishers 2004