Biological Laser Printing: A Novel Technique for Creating Heterogeneous 3-dimensional Cell Patterns
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We have developed a laser-based printing technique, called biological laser printing (BioLP™). BioLP is a non-contact, orifice-free technique that rapidly deposits fL to nL scale volumes of biological material with spatial accuracy better than 5 μm. The printer's orifice-free nature allows for transfer of a wide range of biological material onto a variety of substrates. Control of transfer is performed via a computer-aided design/computer-aided manufacturing (CAD/CAM) system which allows for deposition rates up to 100 pixels of biological material per second using the current laser systems. In this article, we present a description of the apparatus, a model of the transfer process, and a comparison to other biological printing techniques. Further, examples of current system capabilities, such as adjacent deposition of multiple cell types, large-scale cell arrays, and preliminary experiments on creating multi-layer cell constructs are presented. These cell printing experiments not only demonstrate near 100% viability, they also are the first steps toward using BioLP to create heterogeneous 3-dimensional constructs for use in tissue engineering applications.
- J.A. Barron, B.J. Spargo, and B.R. Ringeisen, Applied Physics A, in press (2004).
- S. Belkin, Current Opinions in Microbiology 6, 206 (2003).
- S. Belkin, T.K. Van Dyk, A.C. Vollmer, D.R. Smulski, and R.A. LaRossa, Environmental Toxicology and Water Quality 11, 179 (1996).
- A. Bruckbauer, L.M. Ying, A.M. Rothery, D.J. Zhou, A.I. Shevchuk, C. Abell, Y.E. Korchev, and D. Klenerman, Journal of the American Chemical Society 124, 8810 (2002).
- M.V. Catani, A. Rossi, A. Costanzo, S. Sabatini, M. Levrero, G. Melino, and L. Avigliano, Biochemical Journal 356, 77 (2001).
- M.S. Chapekar, Journal of Biomedical Materials Research 53, 617 (2000).
- Y. Dou, L.V. Zhigilei, A. Postawa, N. Winograd, and B.J. Garrison, Nuclear Instruments and Methods in Physics Research B 180, 105 (2001).
- H.A. Fishman, O. Orwar, R.H. Scheller, and R.N. Zare, Proceedings of the National Academy of Sciences of the United States of America 92, 7877 (1995).
- L.G. Griffith and G. Naughton, Science 295, 1009 (2002).
- P. Gwynne and G. Heebner, Drug Discovery and Biotechnology Trends: Proteomics 2: Microarrays, the Next Step, Science E-Marketplace, http://www.sciencemag.org/feature/e-market/benchtop/ddbt_10303.shl (2003a).
- P. Gwynne and G. Heebner, Drug Discovery and Biotechnology Trends: Analysis and Separation: DNA and Biochips 1, Science E-Marketplace, http://www.sciencemag.org/feature/e-market/benchtop/ddbd_0314.shl (2003b).
- J. Hyun, S.J. Ahn, W.K. Lee, A. Chilkoti, and S. Zauscher, Nanoletters 2, 1203 (2002).
- R.S. Kane, S. Takayama, E. Ostuni, D.E. Ingber, and G.M. Whitesides, Biomaterials 20, 2363 (1999).
- S. Kohler, S. Belkin, and R.D. Schmid, Fresenius Journal of Analytical Chemistry 366, 769 (2000).
- K.B. Lee, J.H. Lim, and C.A. Mirkin, Journal of the American Chemical Society 125, 5588 (2003).
- K.B. Lee, S.J. Park, C.A. Mirkin, J.C. Smith, and M. Miksich, Science 295, 1702 (2002).
- W. Malomi, E. Straface, G. Donelli, and P.U. Giacomoni, European Journal of Dermatology 6, 414 (1996).
- V. Mironov, T. Boland, T. Trusk, G. Forgacs, and R.R. Markwald, Trends in Biotechnology 21, 157 (2003).
- E. Palik, Handbook of Optical Constants of Solids (Academic Press, New York, 1985).
- W.H. Parkinson and K. Yoshino, Chemical Physics 294, 31 (2003).
- B.R. Ringeisen, J.A. Barron, and D.B. Krizman, in Protein Microarrays, edited by P. Schena (Jones and Bartlett, Boston, 2004).
- B.R. Ringeisen, H. Kim, J.A. Barron, D.B. Krizman, D.B. Chrisey, S. Jackman, R.Y.C. Anyeung, and B.J. Spargo, Tissue Engineering, in press (2004).
- B.R. Ringeisen, P.K. Wu, H. Kim, A. Pique, R.Y.C. Auyeung, H.D. Young, and D.B. Chrisey, Biotechnology Progress 18, 1126 (2002).
- A. Roda, M. Guardigli, C. Russo, P. Pasini, and M. Baraldini, Biotechniques 28, 492 (2000).
- R.S. Takayama, E. Ostuni, D.E. Ingber, and G.M. Whitesides, Biomaterials 20, 2363 (1999).
- C. Vedrine, J.C. Leclerc, C. Durrieu, and C. Tran-Minh, Biosensors and Bioelectronics 18, 457 (2003).
- A.C. Vollmer, S. Belkin, D.R. Smulski, T.K. Van Dyk, and R.A. LaRossa, Applied and Environmental Microbiology 63, 2566 (1997).
- W.C Wilson and T. Boland, Anatomical Record Part A-Discoveries in Molecular, Cellular and Evolutionary Biology 272A, 491 (2003).
- P.K Wu, B.R. Ringeisen, D.B. Krizman, C.G. Frondoza, M. Brooks, D.M. Bubb, R.C.Y. Auyeung, A. Pique, B. Sparge, R.A. McGill, and D.B. Chrisey, Review of Scientific Instruments 74, 2546 (2003).
- Biological Laser Printing: A Novel Technique for Creating Heterogeneous 3-dimensional Cell Patterns
Volume 6, Issue 2 , pp 139-147
- Cover Date
- Print ISSN
- Online ISSN
- Kluwer Academic Publishers
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- biological laser printing (BioLP)
- cell printing
- cell patterning
- tissue engineering
- Industry Sectors