Biomedical Microdevices

, Volume 6, Issue 1, pp 75–80

Surface Effects on PCR Reactions in Multichip Microfluidic Platforms

Authors

  • Nicholas J. Panaro
    • Department of Pathology and Laboratory MedicineHospital of the University of Pennsylvania
  • Xing Jian Lou
    • Department of Pathology and Laboratory MedicineHospital of the University of Pennsylvania
  • Paolo Fortina
    • Center for Translational Medicine, Department of Medicine, Jefferson Medical CollegeThomas Jefferson University
    • Department of Pathology and Laboratory MedicineHospital of the University of Pennsylvania
  • Peter Wilding
    • Department of Pathology and Laboratory MedicineHospital of the University of Pennsylvania
Article

DOI: 10.1023/B:BMMD.0000013369.34716.8d

Cite this article as:
Panaro, N.J., Lou, X.J., Fortina, P. et al. Biomedical Microdevices (2004) 6: 75. doi:10.1023/B:BMMD.0000013369.34716.8d

Abstract

We evaluated the compatibility of several common plastics, commercially available plastic tubing and disposable syringes which might be useful in the construction of microfluidic platforms with respect to the polymerase chain reaction (PCR). A simple and inexpensive plastic test module was constructed in order to evaluate some of the construction plastics. We also investigated the effect of addition of PEG 8000 to PCR reaction mixtures on the compatibility of materials. These studies identified several common plastics, plastic tubing, and disposable syringes which were compatible with the PCR reaction.

polymerase chain reactionlab on a chipmicrofluidicsmicrofluidic platformmaterials compatibility

Copyright information

© Kluwer Academic Publishers 2004