Article

Biomedical Microdevices

, Volume 6, Issue 1, pp 75-80

Surface Effects on PCR Reactions in Multichip Microfluidic Platforms

  • Nicholas J. PanaroAffiliated withDepartment of Pathology and Laboratory Medicine, Hospital of the University of Pennsylvania
  • , Xing Jian LouAffiliated withDepartment of Pathology and Laboratory Medicine, Hospital of the University of Pennsylvania
  • , Paolo FortinaAffiliated withCenter for Translational Medicine, Department of Medicine, Jefferson Medical College, Thomas Jefferson University
  • , Larry J. KrickaAffiliated withDepartment of Pathology and Laboratory Medicine, Hospital of the University of Pennsylvania Email author 
  • , Peter WildingAffiliated withDepartment of Pathology and Laboratory Medicine, Hospital of the University of Pennsylvania

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Abstract

We evaluated the compatibility of several common plastics, commercially available plastic tubing and disposable syringes which might be useful in the construction of microfluidic platforms with respect to the polymerase chain reaction (PCR). A simple and inexpensive plastic test module was constructed in order to evaluate some of the construction plastics. We also investigated the effect of addition of PEG 8000 to PCR reaction mixtures on the compatibility of materials. These studies identified several common plastics, plastic tubing, and disposable syringes which were compatible with the PCR reaction.

polymerase chain reaction lab on a chip microfluidics microfluidic platform materials compatibility