Biotechnology Letters

, Volume 26, Issue 20, pp 1593-1594

First online:

Improved long-term cryostorage of Escherichia colicompetent cells using trehalose

  • Taeho AhnAffiliated withDepartment of Biochemistry, College of Veterinary Medicine
  • , Seong Su KangAffiliated withInterdisciplinary Program of Brain Science, Chonnam National University
  • , Chul-Ho YunAffiliated withSchool of Biological Sciences and Technology, Chonnam National University

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Trehalose added to storage buffers for chemical-induced transformations at 200 mm increased the survival of Escherichia coli competent cells 1.3∼4.8-fold over 180 d cryostorage compared with dimethyl sulfoxide or glycerol.

competent Escherichia coli transformation trehalose