, Volume 26, Issue 20, pp 1593-1594

Improved long-term cryostorage of Escherichia colicompetent cells using trehalose

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Abstract

Trehalose added to storage buffers for chemical-induced transformations at 200 mm increased the survival of Escherichia coli competent cells 1.3∼4.8-fold over 180 d cryostorage compared with dimethyl sulfoxide or glycerol.