Biotechnology Letters

, Volume 26, Issue 19, pp 1487–1491

Production of mevalonate by a metabolically-engineered Escherichia coli

  • Authors
  • Kazuhiko Tabata
  • Shin-Ichi Hashimoto

DOI: 10.1023/B:BILE.0000044449.08268.7d

Cite this article as:
Tabata, K. & Hashimoto, S. Biotechnology Letters (2004) 26: 1487. doi:10.1023/B:BILE.0000044449.08268.7d


Mevalonate is biosynthesized from acetyl-CoA and metabolized to isoprenoid compounds in a wide variety of organisms although certain types of prokaryotes employ another route for isoprenoid biosynthesis (the non-mevalonate pathway). To establish a fermentative process for mevalonate production, enzymes for mevalonate synthesis from Enterococcus faecalis were expressed in Escherichia coli, a non-mevalonate pathway bacterium. Mevalonate was accumulated, indicating a redirection of acetate metabolism by the expressed enzyme. The recombinant E. coli produced 47 g mevalonate l−1 in 50 h of fed-batch cultivation in a 2 l jar fermenter; this is the highest titer ever reported demonstrating the superiority of E. coli in its ability of acetyl-CoA supply and its inability is degrade mevalonate.

acetatefed-batch fermentationmevalonatemevalonate pathwaynon-mevalonate pathway

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© Kluwer Academic Publishers 2004