Regioselective acylation of flavonoids catalyzed by immobilized Candida antarctica lipase under reduced pressure
10.1023/B:BILE.0000032967.23282.15 Cite this article as: Passicos, E., Santarelli, X. & Coulon, D. Biotechnology Letters (2004) 26: 1073. doi:10.1023/B:BILE.0000032967.23282.15 Abstract
A single-step acylation of rutin and naringin, catalyzed by immobilized
Candida antarctica lipase B in 2-methyl-2-butanol, occurred preferentially on the primary hydroxyl group. Using palmitic methyl ester as acyl donor, the acylation rate of naringin was 10-fold higher than that of rutin. Under optimal conditions, i.e. a molar ratio acyl donor/naringin of 7:1 and 200 mbar, 92% naringin was acylated. Candida antarctica lipase flavonoid ester molar ratio reduced pressure transesterification References
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