Article

Biotechnology Letters

, Volume 26, Issue 13, pp 1057-1059

Scale-up production of puerarin from hairy roots of Pueraria phaseoloides in an airlift bioreactor

  • Spiridon KintziosAffiliated withLaboratory of Plant Physiology, Faculty of Agricultural Biotechnology, Agricultural, University of Athens
  • , Olga MakriAffiliated withLaboratory of Plant Physiology, Faculty of Agricultural Biotechnology, Agricultural, University of Athens
  • , Eleni PistolaAffiliated withLaboratory of Plant Physiology, Faculty of Agricultural Biotechnology, Agricultural, University of Athens
  • , Theodoros MatakiadisAffiliated withLaboratory of Plant Physiology, Faculty of Agricultural Biotechnology, Agricultural, University of Athens
  • , He Ping ShiAffiliated withCollege of Life Science, South China Normal University
  • , Athanassios EconomouAffiliated withLaboratory of Floriculture, Aristotle University

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Abstract

Hairy roots of the Chinese herb, Pueraria phaseoloides, obtained from leaf explants and transformed with the Agrobacterium rhizogenes, were cultured in 2.5 l airlift bioreactors for three weeks. Puerarin accumulated at 5570 μg g−1 dry wt, which is near 200 times as much as in 250 ml flask cultures. In addition, puearin was exuded into the nutrient medium at final concentrations higher than in the hairy roots themselves.

airlift bioreactor Pueraria phaseoloides puerarin transformed roots