Biotechnology Letters

, Volume 26, Issue 13, pp 1057–1059

Scale-up production of puerarin from hairy roots of Pueraria phaseoloides in an airlift bioreactor

Authors

  • Spiridon Kintzios
    • Laboratory of Plant Physiology, Faculty of Agricultural Biotechnology, AgriculturalUniversity of Athens
  • Olga Makri
    • Laboratory of Plant Physiology, Faculty of Agricultural Biotechnology, AgriculturalUniversity of Athens
  • Eleni Pistola
    • Laboratory of Plant Physiology, Faculty of Agricultural Biotechnology, AgriculturalUniversity of Athens
  • Theodoros Matakiadis
    • Laboratory of Plant Physiology, Faculty of Agricultural Biotechnology, AgriculturalUniversity of Athens
  • He Ping Shi
    • College of Life ScienceSouth China Normal University
  • Athanassios Economou
    • Laboratory of FloricultureAristotle University
Article

DOI: 10.1023/B:BILE.0000032963.41208.e8

Cite this article as:
Kintzios, S., Makri, O., Pistola, E. et al. Biotechnology Letters (2004) 26: 1057. doi:10.1023/B:BILE.0000032963.41208.e8

Abstract

Hairy roots of the Chinese herb, Pueraria phaseoloides, obtained from leaf explants and transformed with the Agrobacterium rhizogenes, were cultured in 2.5 l airlift bioreactors for three weeks. Puerarin accumulated at 5570 μg g−1 dry wt, which is near 200 times as much as in 250 ml flask cultures. In addition, puearin was exuded into the nutrient medium at final concentrations higher than in the hairy roots themselves.

airlift bioreactorPueraria phaseoloidespuerarintransformed roots
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© Kluwer Academic Publishers 2004