Biotechnology Letters

, Volume 26, Issue 8, pp 645–647

Biosensor based on Langmuir–Blodgett films of poly(3-hexyl thiophene) for detection of galactose in human blood

  • Sandeep K. Sharma
  • Rahul Singhal
  • B.D. Malhotra
  • Neeta Sehgal
  • Ashok Kumar
Article

DOI: 10.1023/B:BILE.0000023023.19651.01

Cite this article as:
Sharma, S.K., Singhal, R., Malhotra, B. et al. Biotechnology Letters (2004) 26: 645. doi:10.1023/B:BILE.0000023023.19651.01

Abstract

An amperometric biosensor was developed to estimate galactose in human blood serum. Monolayers of poly(3-hexyl thiophene) were placed on glass plates coated with indium tin oxide formed by dispensing a mixed solution of stearic acid in chloroform on to a water sub-phase. Galactose oxidase was mixed with poly(3-hexyl thiophene)/stearic acid in chloroform and dispensed on to the air-water interface of Langmuir–Blodgett trough. These monolayers were transferred on to glass plates which were used as working electrodes with platinum as a reference electrode. The amperometric galactose biosensor thus fabricated had a linear response from 0.05 to 0.5 g galactose l−1 in blood serum. The normal level in blood is < 0.05 g galactose l−1 in adults and 0–0.2 g galactose l−1 in infants. In case of galactosemia, this increases to above 0.2 g galactose l−1 in infants.

biosensorgalactosegalactosemiaLangmuir–Blodgett filmspoly(3-hexyl thiophene)

Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • Sandeep K. Sharma
    • 1
  • Rahul Singhal
    • 2
  • B.D. Malhotra
    • 2
  • Neeta Sehgal
    • 3
  • Ashok Kumar
    • 1
  1. 1.Institute of Genomics and Integrative BiologyDelhiIndia
  2. 2.National Physical LaboratoryNew DelhiIndia
  3. 3.Department of ZoologyUniversity of DelhiDelhiIndia