Biotechnology Letters

, Volume 26, Issue 8, pp 629–633

Enhanced activity and enantioselectivity of Candida rugosa lipase immobilized on macroporous adsorptive resins for ibuprofen resolution

  • Hongwei Yu
  • Jinchuan Wu
  • Chi Bun Ching
Article

DOI: 10.1023/B:BILE.0000023020.12576.1e

Cite this article as:
Yu, H., Wu, J. & Ching, C.B. Biotechnology Letters (2004) 26: 629. doi:10.1023/B:BILE.0000023020.12576.1e

Abstract

The lipase from Candida rugosa was immobilized on three commercially available macroporous adsorptive resins for kinetic resolution of ibuprofen. One resin, CRB02, increased the enzyme activity by 50% to 0.027 g g−1 min−1. The deactivation constant (0.19 h−1) of the immobilized enzyme was half of that of the native enzyme and the enantioselectivity (E = 29.2) of the immobilized lipase was 2.2 times as much as that of the native lipase for the kinetic resolution of ibuprofen with 1-propanol in isooctane at 30 °C.

Candida rugosa ibuprofen immobilization lipase macroporous adsorptive resin resolution 

Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • Hongwei Yu
    • 1
  • Jinchuan Wu
    • 2
  • Chi Bun Ching
    • 1
  1. 1.Chemical and Process Engineering CentreNational University of SingaporeSingapore
  2. 2.Institute of Chemical Engineering SciencesSingapore

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