Biochemical Genetics

, Volume 42, Issue 7, pp 231–240

Gene Frequency Distribution of the BoLA-DRB3 Locus in Saavedreño Creole Dairy Cattle

  • M. V. Ripoli
  • J. P. Lirón
  • J. C. De Luca
  • F. Rojas
  • F. N. Dulout
  • G. Giovambattista
Article

DOI: 10.1023/B:BIGI.0000034427.66887.fe

Cite this article as:
Ripoli, M.V., Lirón, J.P., De Luca, J.C. et al. Biochem Genet (2004) 42: 231. doi:10.1023/B:BIGI.0000034427.66887.fe

Abstract

The objective of this study is to describe the gene frequency distribution of the bovine lymphocyte antigen (BoLA)-DRB3 locus in Saavedreño Creole dairy cattle and to compare it with previously reported patterns in other cattle breeds. One hundred and twenty-five Saavedreño Creole dairy cattle were genotyped for the BoLA-DRB3.2 allele by polymerase chain reaction and restriction fragment length polymorphism. Twenty-two out of 53 previously identified BoLA-DRB3.2 alleles were detected, with gene frequencies ranging from 0.4 to 16.8%. Seventy percent of the variation corresponded to the seven most frequent alleles (BoLA-DRB3.2*7, *8, *11, *16, *27, *36, and *37). The studied population exhibits a high degree of expected heterozygosity (he = 0.919). The FIS index did not show significant deviation from Hardy-Weinberg equilibrium. However, the neutrality test showed an even gene frequency distribution. This result could be better explained assuming balancing selection instead of neutral or positive selection for one or a few alleles. In conclusion, the results of this study demonstrated that BoLA-DRB3.2 is a highly polymorphic locus in Saavedreño Creole dairy cattle, with significant variation in allele frequency among cattle breeds.

BoLA-DRB3.2Creole cattlePCR-RFLPgenetic diversity

Copyright information

© Plenum Publishing Corporation 2004

Authors and Affiliations

  • M. V. Ripoli
    • 1
  • J. P. Lirón
    • 1
  • J. C. De Luca
    • 1
  • F. Rojas
    • 2
  • F. N. Dulout
    • 1
  • G. Giovambattista
    • 1
  1. 1.Centro de Investigaciones en Genética Básica y Aplicada (CIGEBA), Facultad de Ciencias VeterinariasUniversidad Nacional de La PlataArgentina
  2. 2.Av. Ejercito Nacional 131Santa Cruz de la SierraBolivia