Regeneration and multiplication of Albizia procera Benth. through organogenesis
Rent the article at a discountRent now
* Final gross prices may vary according to local VAT.Get Access
Vegetative propagation techniques are recognized as indispensable tools for mass multiplication of important multipurpose trees adopted in different agroforestry systems. Albizia procera, one among important species, is difficult to propagate commercially either by stem / root cuttings or layering. A study was undertaken to develop procedure for its in vitro regeneration through organogenesis. Explants collected from 15±2 yr-old mature plus trees and from 15 days old juvenile seedlings were regenerated with exogenous application of different hormones. Epicotyl and hypocotyl explants excised from juvenile seedlings showed higher callusing than axillary bud and shoot tip explants derived from mature trees. Benzylaminopurine (BA) at 3 µg/l was most effective, which induced hundred percent callusing in epicotyl and hypocotyl explants in 1/2 Murashige and Skoog (MS) medium. Callus originated from axillary buds and apical shoot tips of mature trees failed to form organs, however callus derived from epicotyl and hypocotyl explants proliferated and formed de novo shoots and leaflets. A concentration of 3 µg/l of BA was found effective for shoot proliferation. Shoots grew vigorously in 2 µg/l gibberellic acid (GA3) treatment and rooted in 1/2 MS medium supplemented with indole-3-butyric acid (IBA) and indole-3-acetic acid (IAA). Rooting was most successful on medium supplemented with 6 µg/l IBA alone on which 93.3% of the shoots formed roots. Sand or vermiculite supplemented with 4 ml of yoshida solution proved as best hardening media, which recorded 70-80% survival of plantlets. One year old tissue culture raised plants had comparatively more height, collar diameter, biomass, and root shoot ratio than plants raised from cuttings and seeds of the same age. The procedures enumerated provide a basis for the development of in vitro techniques for rapid multiplication of A. procera.
- Ansari S.A., Kumar P. and Mandal A.K. 1998. An airlayering method for clonal propagation of Albizia procera. Indian Forester 124(5): 354–356.
- Bhau B.S. and Wakhlu A.K. 2001. Effect of genotype, explant type and growth regulators on organogenesis in Morus alba. Plant Cell Tissue and Organ Culture 66: 25–29. CrossRef
- Bist P., Joshi I., Chauhan J.M.S. and Sharma V.K. 2000. In vitro clonal propagation of mature Eucalyptus F1 hybrid FRI-5 (E. camandulensis Dehn. X E. teriticornis Sm.). Indian Journal of Forestry 25(1): 28–32.
- Druart P., Kevers C., Boxus P. and Gaspar T. 1982. In vitro promotion of root formation by apple shoots through darkness effect on endogenous phenols and peroxidases. Zeitschrift fur Pflanzenphysiologie 108: 429–436.
- Dumas E. and Monteuuis O. 1995. In vitro rooting of micropropagated shoots from juvenile and mature Pinus pinaster explants: influence of activated charcoal. Plant Cell Tissue and Organ Culture 40: 231–235. CrossRef
- Duncan D.B. 1955. Multiple range test and multiple F test. Biometrics 11: 1–48. CrossRef
- Ganguli J.L., Yadav S.B., Swamy S.L. and Puri S. 2000. Bruchus bilineatopygus Pic. A pest of Albizia procera and A. lebbeck seeds in storage. Insect environment 6(1): 11–13.
- George E.F. 1993. Plant Propagation by Tissue Culture. Exergetics Ltd., Basingstoke, UK.
- Gharyal P.K. and Maheshwari S.C. 1990. Differentiation in explants from mature leguminous trees. Plant Cell Reports 8: 550–553. CrossRef
- Gomez K.A. and Gomez A.A. 1984. Statistical Procedures for Agricultural Research. John Wiley and Sons, New York, USA.
- Jordan M., Larrain M., Tapra A. and Rouer R. 2001. In vitro regeneration of Sophora toromiro from seedling explants. Plant Cell Tissue and Organ Culture 66: 89–95. CrossRef
- Kanwar K., Sehgal R.N. and Sood D. 1995. Effect of explant type on the micropropagation of Robinia pseudoacacia. Indian Journal of Forestry 18(1): 47–52.
- Kathiravan K., Ganapathi A. and Shajahan A. 1997. Adventitious shoot formation and plant regeneration from callus cultures of mulberry (Morus alba). Sericologia 37: 727–733.
- Kumar S., Sarkar A.K. and Kunhi Kannan 1998. Regeneration of plants from leaflet explants of tissue culture raised safed siris (Albizia procera). Plant Cell Tissue and Organ Culture 54: 137–143. CrossRef
- Leakey R.R.B., Newton A.C. and Dick J.M.P. 1994. Capture of genetic variation by vegetative propagation: processes determining success. In: Leakey R.R.B. and Newton A.C. (eds), Tropical trees: The potential for domestication and rebuilding of forest resources pp 72–83. HMSO, London, UK.
- Luna R.K. 1996. Plantation Trees. International Book Distributors, Rajpur Road, Dehra Dun, India.
- Monteuuis O. and Bon M.C. 1986. Microbourt urage du sequoia grant. Ann. AFOCEL 1985. pp. 49–87.
- Murashige T. and Skoog F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum 15: 473–497. CrossRef
- Nouaim R., Mangin G., Beruil M.C. and Chaussod R. 2002. The argan tree (Argania spinosa) in Morocco: Propagation by seeds, cuttings and in vitro techniques. Agroforestry Systems 54: 71–81. CrossRef
- Poupard C., Chauviere M. and Monteuuis O. 1994. Rooting Acacia mangium cuttings: effects of age, within-shoot position and auxin treatment. Silvae Genetica 43(4): 226–231.
- Roy A.T. 1992. In vitro propagation of Albizia lebbeck using axillary and apical buds. pp 9–17. In: Dhawan V., Ganapathy P.M. and Khurana D.K. (eds), Tissue culture of forest species: recent researches in India. Proc. of National Workshop on mass propagation of tree species through in vitro methods. New Delhi, India.
- Swamy S.L., Puri S. and Singh A.K. 2002. Effect of auxins (IBA and NAA) and season on rooting of juvenile and mature hardwood cuttings of Robinia pseudoacacia and Grewia optiva. New Forests 23: 143–157. CrossRef
- Swamy S.L., Puri S. and Yadav S.B. 2000. Propagation of Albizia procera Benth. using cutting and air layering techniques. Indian Journal of Agroforestry 1(2): 149–152.
- Regeneration and multiplication of Albizia procera Benth. through organogenesis
Volume 60, Issue 2 , pp 113-121
- Cover Date
- Print ISSN
- Online ISSN
- Kluwer Academic Publishers
- Additional Links
- Tissue culture
- Vegetative propagation
- Industry Sectors