Biomedical Microdevices

, Volume 5, Issue 4, pp 281–290

Poly(dimethylsiloxane) (PDMS) and Silicon Hybrid Biochip for Bacterial Culture

Authors

  • Woo-Jin Chang
    • Laboratory of Integrated Biomedical Micro/Nanotechnology and ApplicationsBirck Nanotechnology Center, School of Electrical and Computer Engineering and the Department of Biomedical Engineering
  • Demir Akin
    • Laboratory of Integrated Biomedical Micro/Nanotechnology and ApplicationsBirck Nanotechnology Center, School of Electrical and Computer Engineering and the Department of Biomedical Engineering
  • Miroslav Sedlak
    • Department of Biomedical Engineering
    • Department of Agricultural and Biological EngineeringPurdue University
  • Michael R. Ladisch
    • Department of Biomedical Engineering
    • Department of Agricultural and Biological EngineeringPurdue University
  • Rashid Bashir
    • Laboratory of Integrated Biomedical Micro/Nanotechnology and ApplicationsBirck Nanotechnology Center, School of Electrical and Computer Engineering and the Department of Biomedical Engineering
Article

DOI: 10.1023/A:1027301628547

Cite this article as:
Chang, W., Akin, D., Sedlak, M. et al. Biomedical Microdevices (2003) 5: 281. doi:10.1023/A:1027301628547

Abstract

In this study, a novel PDMS/silicon hybrid microfluidic biochip was fabricated and tested for the long-term batch culture of bacterial cells. The PDMS (poly(dimethylsiloxane)) cover with 3-dimensional micro-channels for flow was fabricated using Teflon tubing and hole-punch techniques, without photolithographic methods. The PDMS/silicon hybrid biochip was prepared by bonding of PDMS cover and a silicon chip that had electrodes and micro-fluidic channels defined. The absorption of liquid into PDMS cover was characterized and conditions to prevent drying of nutrient media within the micro-chamber were shown. The absorption of liquid from micro-chambers into the PDMS cover was reduced up to 2.5 times by changing the mixing ratio of PDMS and curing agent from 10 : 1 to 2.5 : 1. In addition, pre-saturation of the PDMS cover with media prior to the incubation resulted in the preservation of liquid in the micro-chambers for up to 22 hours. Optimization of the mixing ratio and pre-saturation of the PDMS cover reduced the drying time 10 times when compared to the unsaturated PDMS cover composed of 10 : 1 ratio of PDMS and curing agent. Listeria innocua and a strain of Escherichia coli, expressing green fluorescent protein (GFP), were successfully cultured in batch mode within the PDMS/silicon hybrid biochip.

PDMS/silicon hybrid biochipbacterial culturefabrication of 3-dimensional micro-channelabsorption in PDMS

Copyright information

© Kluwer Academic Publishers 2003