Biomedical Microdevices

, Volume 5, Issue 4, pp 281–290

Poly(dimethylsiloxane) (PDMS) and Silicon Hybrid Biochip for Bacterial Culture

  • Woo-Jin Chang
  • Demir Akin
  • Miroslav Sedlak
  • Michael R. Ladisch
  • Rashid Bashir
Article

DOI: 10.1023/A:1027301628547

Cite this article as:
Chang, W., Akin, D., Sedlak, M. et al. Biomedical Microdevices (2003) 5: 281. doi:10.1023/A:1027301628547

Abstract

In this study, a novel PDMS/silicon hybrid microfluidic biochip was fabricated and tested for the long-term batch culture of bacterial cells. The PDMS (poly(dimethylsiloxane)) cover with 3-dimensional micro-channels for flow was fabricated using Teflon tubing and hole-punch techniques, without photolithographic methods. The PDMS/silicon hybrid biochip was prepared by bonding of PDMS cover and a silicon chip that had electrodes and micro-fluidic channels defined. The absorption of liquid into PDMS cover was characterized and conditions to prevent drying of nutrient media within the micro-chamber were shown. The absorption of liquid from micro-chambers into the PDMS cover was reduced up to 2.5 times by changing the mixing ratio of PDMS and curing agent from 10 : 1 to 2.5 : 1. In addition, pre-saturation of the PDMS cover with media prior to the incubation resulted in the preservation of liquid in the micro-chambers for up to 22 hours. Optimization of the mixing ratio and pre-saturation of the PDMS cover reduced the drying time 10 times when compared to the unsaturated PDMS cover composed of 10 : 1 ratio of PDMS and curing agent. Listeria innocua and a strain of Escherichia coli, expressing green fluorescent protein (GFP), were successfully cultured in batch mode within the PDMS/silicon hybrid biochip.

PDMS/silicon hybrid biochipbacterial culturefabrication of 3-dimensional micro-channelabsorption in PDMS

Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • Woo-Jin Chang
    • 1
  • Demir Akin
    • 1
  • Miroslav Sedlak
    • 2
    • 3
  • Michael R. Ladisch
    • 2
    • 3
  • Rashid Bashir
    • 1
  1. 1.Laboratory of Integrated Biomedical Micro/Nanotechnology and ApplicationsBirck Nanotechnology Center, School of Electrical and Computer Engineering and the Department of Biomedical EngineeringUSA
  2. 2.Department of Biomedical EngineeringUSA
  3. 3.Department of Agricultural and Biological EngineeringPurdue UniversityWest Lafayette