Chromosome Research

, Volume 8, Issue 8, pp 737–746

Gene density in the Giemsa bands of human chromosomes

Authors

  • Concetta Federico
    • Dipartimento di Biologia AnimaleUniversity of Catania
  • Letizia Andreozzi
    • Dipartimento di Biologia AnimaleUniversity of Catania
  • Salvatore Saccone
    • Dipartimento di Biologia AnimaleUniversity of Catania
    • Dipartimento di Protezione e Valorizzazione AgroalimentareUniversity of Bologna
  • Giorgio Bernardi
    • Laboratorio di Evoluzione Molecolare, Stazione Zoologica
Article

DOI: 10.1023/A:1026797522102

Cite this article as:
Federico, C., Andreozzi, L., Saccone, S. et al. Chromosome Res (2000) 8: 737. doi:10.1023/A:1026797522102

Abstract

The human genome is formed by isochores belonging to five families, L1, L2, H1, H2 and H3, that are characterized by increasing GC levels and gene concentrations. In-situ hybridization of DNA from different isochore families provides, therefore, information not only on the correlation between isochores and chromosomal bands, but also on the distribution of genes in chromosomes. Three subsets of R(everse) bands were identified: H3+, H3* and H3, that contain large, moderate, and no detectable amounts, respectively, of the gene-richest H2 and H3 isochores, and replicate very early and early, respectively, in S phase of the cell cycle. Here, we investigated the GC levels, replication timings and DNA compaction of G(iemsa) bands. We showed that G bands comprise two different subsets of bands, one of which is predominantly composed of L1 isochores, replicates at the end of the S phase, has a higher DNA compaction relative to H3+ bands and corresponds to the darkest G bands of Francke (1994). In contrast, the other subset is composed of L2 and H1 isochores, has less-extreme properties in replication and composition and corresponds to the less-dark G bands of Francke.

chromatin condensationhuman genomein-situ hybridizationisochoresreplication timing

Copyright information

© Kluwer Academic Publishers 2000