The Histochemical Journal

, Volume 32, Issue 10, pp 625–629

Immunoreactivity of Six Monoclonal Antibodies Directed Against 1,25-dihydroxyvitamin-D3 Receptors in Human Skin

  • Jörg Reichrath
  • Ulf G. Classen
  • Victor Meineke
  • Hector DeLuca
  • Wolfgang Tilgen
  • Anne Kerber
  • Michael F. Holick
Article

DOI: 10.1023/A:1026748824945

Cite this article as:
Reichrath, J., Classen, U.G., Meineke, V. et al. Histochem J (2000) 32: 625. doi:10.1023/A:1026748824945

Abstract

Using confocal laser scanning microscopy, we tested the suitability of five monoclonal mouse antibodies (IVA7E7, IVB12G12, IVG9C11, VD2F12, and VIIID8C12) that had been raised against different domains of the porcine intestinal 1,25-dihydroxyvitamin-D3 receptor (VDR), for the immunohistological detection of VDR in human skin. The VDR immunoreactivity of these antibodies was compared with the well-characterized VDR-staining pattern of the mouse monoclonal antibody 9A7γ raised against chick intestinal VDR. All six antibodies revealed strong nuclear and qualitatively similar immunoreactivity in all cell layers of the viable epidermis. Our data demonstrate that the five mouse monoclonal antibodies are suitable for immunohistochemical detection of VDR in frozen sections. These antibodies show comparable staining patterns in human skin even though they had been raised against different functional domains of the 1,25-dihydroxyvitamin-D3 receptor.

Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • Jörg Reichrath
    • 1
  • Ulf G. Classen
    • 2
  • Victor Meineke
    • 3
  • Hector DeLuca
    • 4
  • Wolfgang Tilgen
    • 1
  • Anne Kerber
    • 1
  • Michael F. Holick
    • 5
  1. 1.Department of DermatologyUniversity of SaarlandHomburg/SaarGermany
  2. 2.Department of PathologyUniversity of SaarlandHomburg/SaarGermany
  3. 3.Institut für RadiobiologieSanitätsakademie der BundeswehrMünchenGermany
  4. 4.Department of BiochemistryUniverstiy of Wisconsin–MadisonMadisonUSA
  5. 5.Vitamin D, Skin and Bone Research Laboratory, Endocrine Section, Department of MedicineBoston University Medical CenterBostonUSA