Chromosome Research

, Volume 9, Issue 1, pp 53–59

Mapping of 13 horse genes by fluorescence in-situ hybridization (FISH) and somatic cell hybrid analysis

  • Gabriella Lindgren
  • Matthew Breen
  • Sophie Godard
  • Ann Bowling
  • James Murray
  • Marcia Scavone
  • Loren Skow
  • Kaj Sandberg
  • Gérard Guérin
  • Matthew Binns
  • Hans Ellegren
Article

DOI: 10.1023/A:1026743700819

Cite this article as:
Lindgren, G., Breen, M., Godard, S. et al. Chromosome Res (2001) 9: 53. doi:10.1023/A:1026743700819

Abstract

We report fluorescence in-situ hybridization (FISH) and somatic cell hybrid mapping data for 13 different horse genes (ANP, CD2, CLU, CRISP3, CYP17, FGG, IL1RN, IL10, MMP13, PRM1, PTGS2, TNFA and TP53). Primers for PCR amplification of intronic or untranslated regions were designed from horse-specific DNA or mRNA sequences in GenBank. Two different horse bacterial artificial chromosome (BAC) libraries were screened with PCR for clones containing these 13 Type I loci, nine of which were found in the libraries. BAC clones were used as probes in dual colour FISH to confirm their precise chromosomal origin. The remaining four genes were mapped in a somatic cell hybrid panel. All chromosomal assignments except one were in agreement with human–horse ZOO-FISH data and revealed new and more detailed information on the equine comparative map. CLU was mapped by synteny to ECA2 while human–horse ZOO-FISH data predicted that CLU would be located on ECA9. The assignment of IL1RN permitted analysis of gene order conservation between HSA2 and ECA15, which identified that an event of inversion had occurred during the evolution of these two homologous chromosomes.

horsephysical mappingtype I markers

Copyright information

© Kluwer Academic Publishers 2001

Authors and Affiliations

  • Gabriella Lindgren
    • 1
  • Matthew Breen
    • 2
  • Sophie Godard
    • 3
  • Ann Bowling
    • 4
  • James Murray
    • 5
    • 6
  • Marcia Scavone
    • 4
  • Loren Skow
    • 7
  • Kaj Sandberg
    • 8
  • Gérard Guérin
    • 3
  • Matthew Binns
    • 2
  • Hans Ellegren
    • 9
  1. 1.Department of Evolutionary BiologyUppsala UniversityUppsalaSweden
  2. 2.Animal Health TrustSuffolkUK
  3. 3.INRA, Centre de Recherche de Jouy-en-JosasLaboratoire de Génétique biochimique et de CytogénétiqueJouy-en-Josas, CedexFrance
  4. 4.Veterinary Genetics Laboratory, School of Veterinary MedicineUniversity of CaliforniaDavisUSA
  5. 5.Department of Animal ScienceUniversity of CaliforniaDavisUSA
  6. 6.Department of Reproduction and Population Health, School of Veterinary MedicineUniversity of CaliforniaDavisUSA
  7. 7.Department of Veterinary Anatomy and Public HealthTexas A & M UniversityTexasUSA
  8. 8.Department of Animal Breeding and GeneticsSwedish University of Agricultural SciencesUppsalaSweden
  9. 9.Department of Evolutionary BiologyUppsala UniversityUppsalaSweden