Biologia Plantarum

, Volume 43, Issue 3, pp 381–386

Induction of Oxidative Stress and Antioxidant Activity by Hydrogen Peroxide Treatment in Tolerant and Susceptible Wheat Genotypes


DOI: 10.1023/A:1026730008917

Cite this article as:
Sairam, R. & Srivastava, G. Biologia Plantarum (2000) 43: 381. doi:10.1023/A:1026730008917


We induced an oxidative stress by means of exogenous hydrogen peroxide in two wheat genotypes, C 306 (tolerant to water stress) and Hira (susceptible to water stress), and investigated oxidative injury and changes in antioxidant enzymes activity. H2O2 treatment caused chlorophyll degradation, lipid peroxidation, decreased membrane stability and activity of nitrate reductase. Hydrogen peroxide increased the activity of antioxidant enzymes, glutathione reductase and catalase. These effects increased with increasing H2O2 concentrations. However, no change was observed in the activity of superoxide dismutase and proline accumulation.

active oxygen speciescatalasechloroplastglutathione reductaselipid peroxidationnitrate reductaseprolinesuperoxide dismutaseTriticum aestivum

Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  1. 1.Division of Plant PhysiologyIndian Agricultural Research InstituteNew Delhi -India e-mail