, Volume 44, Issue 8, pp 1696-1702

Bovine Milk Inhibits Both Adhesion of Helicobacter pylori to Sulfatide and Helicobacter pylori-Induced Vacuolation of Vero Cells

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Abstract

Adhesion of Helicobacter pylori to gastricmucosal cells is an initial important step incolonization and infection. To study adhesion, weinvestigated whether milk inhibits the adhesion ofHelicobacter pylori to sulfatide, an acidicglycosphingolipid that exists in human gastric mucosaand to which Helicobacter pylori adheres. As a measureof functional significance, we also studied whether milkinhibits Helicobacter pylori-induced vacuolation of Verocells. We used sulfatide-coated polystyrene plates andstudied the effect of bovine milk on the adhesion ofHelicobacter pylori to sulfatide. We used Vero cells for Helicobacter pylori -inducedvacuolation. Bovine milk 100- to 200-fold dilutedsignificantly inhibited both adhesion of Helicobacterpylori to sulfatide and Helicobacter pylori-inducedvacuolation in Vero cells. Bovine milk significantlyinhibited adhesion of Helicobacter pylori to MKN-45cells and Lewis b antigen-coated polystyrene plates. Inaddition, these results suggest that bovine milkcontains active substances that inhibit both adhesion ofHelicobacter pylori to mucosa and vacuole formation.Bovine milk may have a protective effect on the gastricmucosa in Helicobacter pylori-associatedgastritis.