, Volume 44, Issue 8, pp 1531-1538

Bombesin Ameliorates Colonic Damage in Experimental Colitis

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Abstract

In the present study we investigated thepossible therapeutic effects of bombesin on anexperimentally induced colitis model in rats.Inflammation of the colon was induced by a singleintracolonic administration of 30 mg of 2,4,6-trinitrobenzene sulfonic acid(TNBS) at 8 cm from the anus. Immediately after theinduction of colitis, some rats were given bombesin (10μg/kg; subcutaneously) three times a day for 14 days, while another group received vehicletreatment. On day 14, the rats were decapitated andplasma carbonyl content and tissue myeloperoxidaselevel, as an index of granulocyte infiltration intointestinal tissue, were determined in order to obtain anobjective evaluation of colonic injury. In the colitisgroup, increased macroscopic damage score, elevated MPOlevel and high plasma carbonyl content, together with the microscopic appearance revealed severeinflammatory changes resembling IBD. Bombesin treatmentattenuated the TNBS-induced colonic damage andstimulated histopathologically apparent mucosalproliferation, suggesting that bombesin may play a role inprotecting gut integrity.