Isogenic Transgenic Homozygous Fish Induced by Artificial Parthenogenesis
Cite this article as: Nam, Y.K., Cho, Y.S. & Kim, D.S. Transgenic Res (2000) 9: 463. doi:10.1023/A:1026596422225 Abstract
As a model system for vertebrate transgenesis, fish have many attractive advantages, especially with respect to the characteristics of eggs, allowing us to produce isogenic, transgenic, homozygous vertebrates by combining with chromosome-set manipulation. Here, we describe the large-scale production of isogenic transgenic homozygous animals using our experimental organism, the mud loach
Misgurnus mizolepis, by the simple process of artificial parthenogenesis in a single generation. These isogenic fish have retained transgenic homozygous status in a stable manner during the subsequent 5 years, and exhibited increased levels of transgene expression. Furthermore, their isogenic nature was confirmed by cloned transgenic homozygous offspring produced via another step of parthenogenic reproduction of the isogenic homozygous transgenic fish. These results demonstrate that a combination of transgenesis and artificial parthenogenesis will make the rapid utilization of genetically pure homozygous transgenic system in vertebrate transgenesis possible. isogenic mud loach parthenogenesis transgenic homozygous References
Baguisi A, Behboodi E, Melican DT, Pollock JS, Destrempes MM, Cammuso C, et al. (1999) Production of goats by somatic cell nuclear transfer.
Nature Biotech (London)
Bercsenyi M, Magyary I, Urbanyi B, Orban L and Horvath L (1998) Hatching out goldfish from common carp eggs: interspecific androgenesis between two cyprinid species.
Bongers ABJ, Abraca JB, Doulabi Z, Eding EH, Komen J and Richter CJJ (1995) Maternal influence on development of androgenetic clones of common carp,
Bongers ABJ, in't Veld EPC, Avo-Hashema K, Bremmer IM, Eding EH, Komen J. et al. (1994) Androgenesis in common carp (
L.) using UV irradiation in a syntheic ovarian fluid and heat shocks.
Caldovic L and Hackett PB (1995) Development of position-independent expression vectors and their transfer into transgenic fish.
Mol Mar Biol Biotechnol
Charreau B, Tesson L, Soulillou J-P, Pourcel C and Anegon I (1996) Transgenesis in rats: technical aspects and models.
Cibelli JB, Stice SL, Golueke PJ, Kane JJ, Jerry J, Blackwell C, et al. (1998) Cloned transgenic calves produced from nonquiescent fetal fibroblasts.
Davisson MT (1997) Rules and guidelines for genetic nomenclature in mice: excerpted version.
Gill III TJ, Smith GJ, Wissler RW and Kunz HW (1989) The rat as an experimental animal.
Gross ML, Kapuscinski AR and Faras AJ (1994) Nest-specific DNA fingerprints of smallmouth bass in lake Opeongo, Ontario.
Trans Am Fish Soc
Iyengar A, Muller F and Maclean N (1996) Regulation and expression of transgenes in fish - a review.
Kim DS, Jo J-Y and Lee TY (1994) Induction of triploidy in mud loach (
) and its effect on gonad development and growth.
Kim DS, Nam YK and Park I-S (1995) Survival and karyological analysis of reciprocal diploid and triploid hybrids between mud loach (
) and cyprinid loach (
Komen J, Bongers ABJ, Richter CJJ, Muiswinkel WB van and Huisman EA (1991) Gynogenesis in common carp (
L.) II. The production of homozygous gynogenetic clones and F1 hybrids.
Nam YK, Jeong CH and Kim DS (1998) A simple clearing method for improved visualization of
Z expression in fish larvae.
Nam YK, Noh CH and Kim DS (1999) Transmission and expression of an integrated reporter construct in three generations of transgenic mud loach (
Powers DA (1989) Fish as model systems.
Purdom CE, Thompson D and Lou YD (1985) Genetic engineering in rainbow trout,
Richardson by suppression of meiotic and mitotic metaphase.
J Fish Biol
Schneike AE, Kind AJ, Ritchie WA, Mycock K, Scott AR, Ritchie M, et al. (1997) Human factor IX transgenic sheep produced by transfer of nuclei from fetal fibroblasts.
Streisinger G, Singer F, Walker C, Knauber D and Dower N (1986) Segregation analyses and gene-centromere distances in zebrafish.
Streisinger G, Walker C, Dower N, Knauber D and Singer F (1981) Production of clones of homozygous diploid zebrafish (
Thompson D and Scott AP (1984) An analysis of recombination data in gynogenetic diploid rainbow trout.
Young WP, Wheeler PA, Fields RD and Thorgaard GH (1996) DNA fingerprinting confirms isogenicity of androgenetically derived rainbow trout lines.
Zhu Z, Hm XU, Li G, Xie Y and He L (1986) Biological effects of human growth hormone gene microinjected into the fertilized eggs of loach
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