Conservation Genetics

, Volume 1, Issue 2, pp 163–168

Cross-species amplification of microsatellite primers in passerine birds

Authors

    • Laboratory of Animal Ecology, Department of BiologyUniversity of Antwerp (UIA)
  • Stefan van Dongen
    • Laboratory of Animal Ecology, Department of BiologyUniversity of Antwerp (UIA)
  • Erik Matthysen
    • Laboratory of Animal Ecology, Department of BiologyUniversity of Antwerp (UIA)
Article

DOI: 10.1023/A:1026587024065

Cite this article as:
Galbusera, P., van Dongen, S. & Matthysen, E. Conserv Genet (2000) 1: 163. doi:10.1023/A:1026587024065

Abstract

Developing species specific microsatellite primers can be avoidedby using existing markers which amplify across species. However,for passerines, such ‘cross-species’ markers are mostly lackingand few guidelines exist for selecting them from the wide rangeof existing markers. Here cross-species amplification tests of 40microsatellite primers in 13 passerine species show an increasein probability of amplification and polymorphism with decreasingphylogenetic distance. Primers which successfully amplified inmany species had a higher chance to be polymorphic. However,since the amplification success, across a broad range of species,of particular primersets remains difficult to predict it iscrucial to identify such markers empirically. Here we describesuch widely applicable bird (passerines) microsatellite markers.

cross-amplificationmicrosatellitepasserine

Copyright information

© Springer Science+Business Media Dordrecht 2000