Plant Molecular Biology

, Volume 44, Issue 4, pp 463–476

Caleosins: Ca2+-binding proteins associated with lipid bodies

Authors

  • Henrik Næsted
    • Institute of Molecular Biology
  • Gitte I. Frandsen
    • Institute of Molecular Biology
  • Guang-Yuh Jauh
    • Institute of Biological ChemistryWashington State University
  • Immaculada Hernandez-Pinzon
    • John Innes Centre
  • Henrik B. Nielsen
    • Institute of Molecular Biology
  • Denis J. Murphy
    • John Innes Centre
  • John C. Rogers
    • Institute of Biological ChemistryWashington State University
  • John Mundy
    • Institute of Molecular Biology
Article

DOI: 10.1023/A:1026564411918

Cite this article as:
Næsted, H., Frandsen, G.I., Jauh, G. et al. Plant Mol Biol (2000) 44: 463. doi:10.1023/A:1026564411918

Abstract

We have previously identified a rice gene encoding a 27 kDa protein with a single Ca2+-binding EF-hand and a putative membrane anchor. We report here similar genes termed caleosins, CLO, in other plants and fungi; they comprise a multigene family of at least five members in Arabidopsis (AtClo1–5). Northern hybridization demonstrated that AtClo2–4 mRNAs levels were low in various tissues, while AtClo1 mRNA levels were high in developing embryos and mature seeds. Analysis of transgenic Arabidopsis plants expressing the GUS reporter under control of the AtClo1 promoter showed strong levels of expression in developing embryos and also in root tip cells. Antibodies raised against AtCLO1 were used to detect caleosin in cellular fractions of Arabidopsis and rapeseed. This indicated that caleosins are a novel class of lipid body proteins, which may also be associated with an ER subdomain.

Arabidopsis thalianacalcium-binding proteincaleosinEF-handgene expressionlipid bodiesvesicles

Copyright information

© Kluwer Academic Publishers 2000