Antonie van Leeuwenhoek

, Volume 78, Issue 2, pp 123–127

Phylogenetic analysis of Bacillus subtilis and related taxa based on partial gyrA gene sequences

Article

DOI: 10.1023/A:1026555830014

Cite this article as:
Chun, J. & Bae, K.S. Antonie Van Leeuwenhoek (2000) 78: 123. doi:10.1023/A:1026555830014

Abstract

Partial gyrA sequences were determined for twelve strains belonging to Bacillus amyloliquefaciens, B. atrophaeus, B. licheniformis, B. mojavensis,B. subtilis subsp. subtilis, B. subtilissubsp. spizizenii and B. vallismortis. The average nucleotide and translated amino acid similarities for the seven type strains were 83.7 and 95.1%, respectively, whereas the corresponding value for the 16S rRNA sequences was 99.1%. All of the type strains were sharply separated; the closest relationship was found between B. atrophaeus and B. mojavensis which shared a nucleotide similarity of 95.8%. Phylogenetic trees were inferred from gyrA nucleotide sequences using the neighbor-joining, Fitch–Margoliash and maximum parsimony algorithms. The test strains were divided into four groups, which generally reflected results previously reported in restriction digest and DNA-DNA hybridization studies. It is concluded from the comparative sequence analysis that the gyrA sequences provide a firm framework for the rapid and accurate classification and identification of Bacillus subtilis and related taxa.

Bacillus subtilisgyrAphylogenytaxonomy

Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  1. 1.Korean Collection for Type CulturesKorea Research Institute of Bioscience and BiotechnologyTaejonRepublic of Korea
  2. 2.Korean Collection for Type CulturesKorea Research Institute of Bioscience and BiotechnologyTaejonRepublic of Korea