Molecular Breeding

, Volume 6, Issue 5, pp 441–449

Mapping candidate genes in Eucalyptus with emphasis on lignification genes

Authors

  • Jean-Marc Gion
    • Programme Arbres et PlantationsCIRAD-Forêt
  • Philippe Rech
    • Programme Arbres et PlantationsCIRAD-Forêt
    • Pôle de Biotechnologie VégétaleUMR CNRS/Université Paul Sabatier no. 5546
  • Jacqueline Grima-Pettenati
    • Pôle de Biotechnologie VégétaleUMR CNRS/Université Paul Sabatier no. 5546
  • Daniel Verhaegen
    • Programme Arbres et PlantationsCIRAD-Forêt
  • Christophe Plomion
    • Laboratoire de Génétique et Amélioration des Arbres ForestiersINRA
Article

DOI: 10.1023/A:1026552515218

Cite this article as:
Gion, J., Rech, P., Grima-Pettenati, J. et al. Molecular Breeding (2000) 6: 441. doi:10.1023/A:1026552515218

Abstract

We used the single-strand conformation polymorphism (SSCP) technique to map eight genes on Eucalyptus urophylla and Eucalyptus grandis linkage maps. These included four genes involved in the common phenylpropanoid pathway (caffeic acid 3-0-methyltransferase, caffeoyl CoA 3-O-methyltransferase, 4-coumarate CoA ligase and phenylalanine ammonia-lyase), two genes involved in the `lignin specific' pathway (cinnamoyl CoA reductase and cinnamyl alcohol dehydrogenase), and two symbiosis regulated genes (EgHypar and EgTubA1). A novel source of variation which affects the SSCP pattern, i.e. the presence or absence of electrophoresis buffer upon loading the samples into the polyacrylamide gel, was found. The placement of these genes on the Eucalyptus maps was carried out using an interspecific hybrid mapping population. This will further facilitate the identification or exclusion of `positional' candidate genes for characterizing quantitative trait loci (QTL) for wood quality and vegetative propagation related traits.

candidate geneEucalyptusligninsmappingsingle-strand conformation polymorphism

Copyright information

© Kluwer Academic Publishers 2000