Constitutive Expression of Soybean Ferritin cDNA Intransgenic Wheat and Rice Results in Increased Iron Levels in Vegetative Tissues but not in Seeds
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We used particle bombardment to produce transgenic wheat and rice plants expressing recombinant soybean ferritin, a protein that can store large amounts of iron. The cDNA sequence was isolated from soybean by RT-PCR and expressed using the constitutive maize ubiquitin-1 promoter. The presence of ferritin mRNA and protein was confirmed in the vegetative tissues and seeds of transgenic wheat and rice plants by northern and western blot analysis, respectively. The levels of ferritin mRNA were similar in the vegetative tissues of both species, but ferritin protein levels were higher in rice. Both ferritin mRNA and protein levels were lower in wheat and rice seeds. ICAP spectrometry showed that iron levels increased only in vegetative tissues of transgenic plants, and not in the seeds. These data indicate that recombinant ferritin expression under the control of the maize ubiquitin promoter significantly increases iron levels invegetative tissues, but that the levels of recombinant ferritin in seeds are not sufficient to increase iron levels significantly over those in the seeds of non-transgenic plants.
- Altpeter F, Vasil V, Srivastava V, Stöger E, Vasil I (1996) Accelerated production of transgenic wheat (Triticum aestivum L.) plants. Plant Cell Rep 16: 12–17.
- Beard J, Burton J, Theil E (1996) Purified ferritin and soybean meal can be sources of iron for treating iron deficiency in rats. Hum Clin Nut 126: 154–160.
- Briat J-F (1996). Roles of ferritins in plants. J Plant Nut 19: 1331–1342.
- Briat J-F, Lobreaux S, Grignon N, Vansuyt G (1999) Regulation of plant ferritin synthesis: how and why? Cell Mol Life Sci 56: 155–166.
- Christensen AH, Quail PH (1996) Ubiquitin promoter-based vectors for high-level expression of selectable and/or screenable marker genes in monocotyledonous plants. Transgenic Res 5: 213–218.
- Deak M, Horvath GV, Davletova S, Torok K, Sass L, Vass I, Barna B, Kiraly Z, Dudits D (1999) Plants ectopically expressing the iron-binding protein ferritin are tolerant to oxidative damage and pathogens. Nature Biotechnol 17: 192–96.
- Dellaporta SL, Wood J, Hicks JB (1984) Maize DNA miniprep. In: Malmberg R, Messsing J, Sussex I (eds) Molecular Biology of Plants: A Laboratory Course Manual, (pp. 36, 37). Cold Spring Harbor Laboratory Press, N.Y.
- Goto F, Yoshihara T, Saiki H (1998) Iron accumulation in tobacco plants expressing soyabean ferritin gene. Transgenic Res 7: 173–180.
- Goto F, Yoshihara T, Shigemoto N, Toki S, Takaiwa F (1999) Iron fortification of rice seed by the soybean ferritin gene. Nature Biotechnol 17: 282–286.
- Harrison P, Arosio P (1996) The ferritins: molecular properties iron storage function and cellular regulation. Biochim Biophys Acta 1275: 161–203.
- Lescure A-M, Proudhon D, Pesey H, Ragland M, Theil E, Briat J-F (1991) Ferritin gene transcription is regulated by iron in soybean cell cultures. Proc Natl Acad Sci USA 88: 8222–8226.
- Lobreaux S, Briat JF (1991) Ferritin accumulation and degradation in different organs of pea (Pisum sativum) during development. Biochem J 274: 601–606.
- Lobreaux S, Yewdall S, Briat J-F, Harrison P (1992) Amino-acid sequence and predicted three-dimensional structure of pea seed (Pisum sativum) ferritin. Biochem J 288: 931–939.
- Lobreaux S, Hardy T, Briat J-F (1993) Abscisic acid is involved in the iron induced synthesis of maize ferritin. EMBO J 12: 651–657.
- Lobreaux S, Thoiron S, Briat J-F (1995) Induction of ferritin synthesis in maize leaves by an iron-mediated oxidative stress. Plant J 8: 443–449.
- Lucca P, Hurrel R, Potrykus I (1999) Genetic engineering approaches to improve the bioavailability and the level of iron in rice grains. General meeting of the Rockefeller Foundation International Program on Rice Biotechnology (abstract), pp. 150.
- Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plant 15: 473–497.
- Proudhon D, Briat JF, Lescure AM (1989) Iron induction of ferritin synthesis in soybean. Plant Physiol 90: 586–590.
- Ragland M, Briat J-F, Gagnon J, Laulhere J-P, Massenet O, Theil E (1990) Evidence for conservation of ferritin sequences among plants animals and for a transit peptide in soybean. J Biol Chem 265: 18339–18344.
- Ragland M, Theil E (1993) Ferritin (mRNA, protein) and iron concentrations during soybean nodule development. Plant Mol Biol 21: 555–560.
- Sambrook J, Fritsch EF, Maniatis T (1989) Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Press, N.Y.
- Stöger E, Williams S, Keen D, Christou P (1999) Constitutive versus seed specific expression in transgenic wheat: temporal and spatial control. Transgenic Res 8: 73–82.
- Sudhakar D, Duc LT, Bong BB, Tinjuangjun P, Maqbool SB, Valdez M, Jefferson R, Christou P (1998) An efficient rice transformation system utilizing mature seed-derived explants and a portable, inexpensive particle bombardment device. Transgenic Res 7: 289–294.
- Theil E (1987) Ferritin: Structure, gene regulation and cellular function in animals, plants and microorganisms. Ann Rev Biochem 56: 289–315.
- Vasil V, Castilo AM, From ME, Vasil IK (1992) Herbicide resistant fertile transgenic wheat plants obtained by microprojectile bombardment of regenerable embryogenic callus. Bio/Technology 10: 667–674.
- Van Wuytswinkel O, Vansuyt G, Grignon N, Fourcroy P, Briat JF (1999) Iron homeostasis alteration in transgenic tobacco overexpressing ferritin. Plant J 17: 93–97.
- Constitutive Expression of Soybean Ferritin cDNA Intransgenic Wheat and Rice Results in Increased Iron Levels in Vegetative Tissues but not in Seeds
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