Plant and Soil

, Volume 226, Issue 2, pp 275–285

Uptake and transport of organic and inorganic nitrogen by arbuscular mycorrhizal fungi

Authors

  • Heidi-Jayne Hawkins
    • Institute of Plant NutritionHohenheim University
  • Anders Johansen
    • Department of Ecology and Molecular BiologyThe Royal Veterinary and Agricultural University
  • Eckhard George
    • Institute of Plant NutritionHohenheim University
Article

DOI: 10.1023/A:1026500810385

Cite this article as:
Hawkins, H., Johansen, A. & George, E. Plant and Soil (2000) 226: 275. doi:10.1023/A:1026500810385

Abstract

New information on N uptake and transport of inorganic and organic N in arbuscular mycorrhizal fungi is reviewed here. Hyphae of the arbuscular mycorrhizal fungus Glomus mosseae (Nicol. and Gerd.) Gerd. and Trappe (BEG 107) were shown to transport N supplied as 15N-Gly to wheat plants after a 48 h labelling period in semi-hydroponic (Perlite), non-sterile, compartmentalised pot cultures. Of the 15N supplied to hyphae in pot cultures over 48 h, 0.2 and 6% was transported to plants supplied with insufficient N or sufficient N, respectively. The increased 15N uptake at the higher N supply was related to the higher hyphal length density at the higher N supply. These findings were supported by results from in vitro and monoxenic studies. Excised hyphae from four Glomus isolates (BEG 84, 107, 108 and 110) acquired N from both inorganic (15NH415NO3, 15NO3 or 15NH4+) and organic (15N-Gly and 15N-Glu, except in BEG 84 where amino acid uptake was not tested) sources in vitro during short-term experiments. Confirming these studies under sterile conditions where no bacterial mineralisation of organic N occurred, monoxenic cultures of Glomus intraradices Schenk and Smith were shown to transport N from organic sources (15N-Gly and 15N-Glu) to Ri T-DNA transformed, AM-colonised carrot roots in a long-term experiment. The higher N uptake (also from organic N) by isolates from nutrient poor sites (BEG 108 and 110) compared to that from a conventional agricultural field implied that ecotypic differences occur. Although the arbuscular mycorrhizal isolates used contributed to the acquisition of N from both inorganic and organic sources by the host plants/roots used, this was not enough to increase the N nutritional status of the mycorrhizal compared to non-mycorrhizal hosts.

arbuscular mycorrhizaDaucus carotaGlomus mosseaeGlomus intraradicesmonoxenic cultureN uptakeTriticum aestivum

Copyright information

© Kluwer Academic Publishers 2000