Journal of Chemical Ecology

, Volume 26, Issue 12, pp 2725–2736

Effect of Two Wheat Cultivars Differing in Hydroxamic Acid Concentration on Detoxification Metabolism in the AphidSitobion avenae

  • Raúl Loayza-Muro
  • Christian C. Figueroa
  • Hermann M. Niemeyer
Article

DOI: 10.1023/A:1026481524896

Cite this article as:
Loayza-Muro, R., Figueroa, C.C. & Niemeyer, H.M. J Chem Ecol (2000) 26: 2725. doi:10.1023/A:1026481524896

Abstract

Hydroxamic acids (Hx) are wheat secondary metabolites conferring resistance for cereals against aphids. The activity of five enzymatic systems were evaluated in the aphid Sitobion avenae reared on the high-Hx wheat cultivar Chagual and the low-Hx wheat cultivar Huayún for 10 generations. Enzyme solutions were prepared from aphid homogenates and assayed for mixed function oxidases (including cytochrome P-450 monooxygenases and NADPH cytochrome c reductase), glutathione S-transferases, esterases, and catalase. Specific activities per aphid individual of cytochrome P-450 monooxygenases, NADPH cytochrome c reductase, glutathione S-transferases, and esterases were significantly increased in wheat cultivars relative to oat (only marginal increase of esterases in Chagual). Aphids fed on cv. Huayún showed an overall higher induction of enzymatic systems than those fed on cv. Chagual. Comparison of these results with reported effects of Hx on detoxifying enzymes in other insects, including aphids, support the hypothesis that these enzymatic pathways play an important role in the detoxification of toxic host-plant secondary metabolites.

Sitobion avenae aphids hydroxamic acids DIMBOA detoxification cytochrome P-450 monooxygenases NADPH cytochrome c reductase glutathione S-transferases esterases catalase 

Copyright information

© Plenum Publishing Corporation 2000

Authors and Affiliations

  • Raúl Loayza-Muro
    • 1
  • Christian C. Figueroa
    • 1
  • Hermann M. Niemeyer
    • 2
  1. 1.Departamento de Ciencias Ecológicas, Facultad de CienciasUniversidad de ChileSantiagoChile
  2. 2.Departamento de Ciencias Ecológicas, Facultad de CienciasUniversidad de ChileSantiagoChile