, Volume 226, Issue 2, pp 171-177

A fluorescent antibody assay for hyphae and glomalin from arbuscular mycorrhizal fungi

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Abstract

Studies on the role of arbuscular mycorrhizal (AM) fungi in soil have been aided by the use of a monoclonal antibody that detects a molecule common to all isolates of these fungi studied to date. The molecule, glomalin, is a glycoprotein that forms on hyphae, but apparently sloughs off and adheres to soil particles or imbedded plastic mesh. An indirect immunofluorescence (IF) assay is described for detection of glomalin on hyphae attached to roots, in roots, on hyphae traps and on the surface of soil aggregates. Small sieves are used to process hyphae attached to roots and soil aggregates. Glomalin on hyphae and glomalin attached to plastic or nylon are assayed on a 1 cm2 section of meshes. Examples of IF assay results are shown and discussed.

This revised version was published online in June 2006 with corrections to the Cover Date.