Plant and Soil

, 226:171

A fluorescent antibody assay for hyphae and glomalin from arbuscular mycorrhizal fungi

  • S. F. Wright

DOI: 10.1023/A:1026428300172

Cite this article as:
Wright, S.F. Plant and Soil (2000) 226: 171. doi:10.1023/A:1026428300172


Studies on the role of arbuscular mycorrhizal (AM) fungi in soil have been aided by the use of a monoclonal antibody that detects a molecule common to all isolates of these fungi studied to date. The molecule, glomalin, is a glycoprotein that forms on hyphae, but apparently sloughs off and adheres to soil particles or imbedded plastic mesh. An indirect immunofluorescence (IF) assay is described for detection of glomalin on hyphae attached to roots, in roots, on hyphae traps and on the surface of soil aggregates. Small sieves are used to process hyphae attached to roots and soil aggregates. Glomalin on hyphae and glomalin attached to plastic or nylon are assayed on a 1 cm2 section of meshes. Examples of IF assay results are shown and discussed.

glycoproteinimmunofluorescencemonoclonal antibodysoil biology

Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • S. F. Wright
    • 1
  1. 1.Soil Microbial Systems LaboratoryUnited States Department of Agriculture–Agricultural Research ServiceBeltsvilleUSA