The Genome of the Lepidopteran Mamestra brassicae has a Vertebrate-like Content of Methyl-cytosine
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Mamestra brassicae genomic DNAs, isolated from larvae and adult tissues and from in vitro cultured CRL-8003 cells, were enzymatically hydrolysed to nucleosides that were separated by HPLC. HPLC analysis showed that 5mC content in cabbage moth larvae, adults and cultured cells was 8.9 ± 0.5, 9.3% ± 0.2 and 10.2% ± 0.4 respectively. Cabbage moth 5mC content results the highest reported till now in insects and it is similar to the typical vertebrate one. Analysis of MspI and HpaII restriction pattern on M. brassicae DNA showed that a portion of its genome was methylated at CpG sites. Moreover, the absence of small digestion products after MspI digestion suggested that CpG are not clustered in the cabbage moth genome. Finally, methylation of repeated DNAs has been studied. Comparison of the restriction pattern of MspI and HpaII after hybridisation with the hobo, mariner, 28S and 5S rDNA probes did not evidence any difference indicating the absence of CpG methylation in all the studied repeated DNAs.
- Achwal, C.W., A.I. Chiara & H.S. Chandra, 1983. Immunochemical evidence for the presence of 5mC, 6mA and 7mG in human, Drosophila and mealybug DNA. FEBS Lett. 158: 353–358.
- Adams, R.L.P., 1996. DNA methylation, pp. 33–66 in Principles of Medical Biology, Vol. 5. JAI Press Inc., New York.
- Bender, J., 1998. Cytosine methylation of repeated sequences in eukaryotes: the role of DNA pairing. Trends Biol. Sci. 23: 252–256.
- Bestor, T.H., 1999. Sex brings transposons and genomes into conflict. Genetica 107: 289–295.
- Bongiorni, S., O. Cintio & G. Prantera, 1999. The relationship between DNA methylation and chromosome imprinting in the coccid Planococcus citri. Genetics 151: 1471–1478.
- Colot, V. & J.L. Rossignol, 1999. Eukaryotic DNA methylation as an evolutionary device. Bioessays 21: 402–411.
- Deobagkar, D.N., K. Muralidharan, S.G. Devare, K.K. Kalghatgi & H.S. Chandra, 1982. DNA methylation. J. Bioscience 4: 513–526.
- Devajyothi, C. & V. Brahmachari, 1992. Detection of CpA methylase in a insect system: characterization and substrate specificity. Mol. Cell. Biochem. 110: 103–111.
- Feil, R. & S. Khosla, 1999. Genomic imprinting in mammals: interplay between chromatin and DNA methylation? Trends Genet. 15: 431–435.
- Field, L.M., 1989. Changes of DNA methylation are associated with loss of insecticide resistance in the peach-potato aphid Myzus persicae. FEBS Lett. 243: 323–327.
- Geiduschek, E.P. & G.P. Tocchini-Valentini, 1988. Transcription by RNA polymerase III. Ann. Rev. Biochem. 57: 873–914.
- Gowher, H., O. Leismann & A. Jeltsch, 2000. DNA of Drosophila melanogaster contains 5–methylcytosine. EMBO J. 19: 6918–6923.
- Jones, P.A. & P.W. Laird, 1999. Cancer epigenetics comes of age. Nat. Genet. 21: 163–167.
- Jones, P.L. & A.P. Wolffe, 1999. Relationship between chromatin organization and DNA methylation in determining gene expression. Semin. Cancer Biol. 9: 339–347.
- Lyko, F., B.H. Ramsahoye & R. Jaenisch, 2000. DNA methylation in Drosophila melanogaster. Nature 408: 538–540.
- Mandrioli, M., 2002. Cytogenetic characterization of telomeres in the holocentric chromosomes of the lepidopteran Mamestra brassicae. Chromosome Res. 9: 279–286.
- Mandrioli, M., G.C. Manicardi & F. Marec, 2003. Cytogenetic and molecular characterization of the MBSAT1 satellite DNA in holokinetic chromosomes of the cabbage moth, Mamestra brassicae (Lepidoptera). Chromosome Res. 11: 51–56.
- Manicardi, G.C., D. Bizzaro, P. Azzoni & U. Bianchi, 1994. Cytological and electrophoretic analysis of DNA methylation in the holocentric chromosomes of Megoura viciae (Homoptera, Aphididae). Genome 37: 625–630.
- Migeon, B.R., 1994. X-chromosome inactivation: molecular mechanism and genetic consequences. Trends Genet. 10: 230–235.
- Miltenburger, H.G. & P. David, 1976. Nuclear polyhedrosis virus replication in permanent cell lines of the cabbage moth (Mamestra brassicae L.). Naturwissenschaften 63: 197–198.
- Nayak, A.R., J.S. Nair, M.V. Hagde, P.K. Ranjekar & U. Pant, 1991. Genome analysis of two mosquito species. Insect Biochem. 21: 803–808.
- O'Neill, R.W.J., M.J. O'Neill & J.A.M. Graves, 1998. Undermethylation is associated with retroelement activation and chromosome remodelling in an interspecific mammalian hybrid. Nature 393: 68–72.
- Patel, C.V. & K.P. Gopinathan, 1987. Determination of trace amounts of 5–methylcytosine in DNA by HPLC. Anal. Biochem. 164: 164–169.
- Sarkar, S., S.R.V. Rao, V.S. Gupta & R. Hendre, 1992. 5–methycytosine content in Gryllotalpa fossor (Orthoptera). Genome 35: 163–166.
- Tate, P.H. & A.P. Bird, 1993. Effects of DNA methylation on DNAbinding proteins and gene expression. Curr. Opin. Genet. Dev. 3: 226–231.
- Toyota, M. & J.P. Issa, 1999. CpG island methylator phenotypes in aging and cancer. Semin. Cancer Biol. 9: 349–357.
- Tweedie, S., J. Charlton, V. Clark & A. Bird, 1997. Methylation of genomes and genes at the invertebrate-vertebrate boundary. Mol. Cell. Biol. 17: 1469–1475.
- Tweedie, S., H.H. Ng, A.L. Barlow, B.M. Turner, B. Hendrich & A. Bird, 1999. Vestiges of a DNA methylation system in Drosophila melonogaster. Nat. Genet. 23: 389–390.
- Urieli-Shoval, S., Y. Gruenbaum, J. Sedat & A. Razin, 1982. The absence of detectable methylated bases in Drosophila melanogaster DNA. FEBS Lett. 146: 148–152.
- Yoder, J., C.P. Walsh & T.H. Bestor, 1997. Cytosine methylation and the ecology of intragenomic parasites. Trends Genet. 13: 335–340.
- The Genome of the Lepidopteran Mamestra brassicae has a Vertebrate-like Content of Methyl-cytosine
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