Abstract
Pollen germination, division of the generative nucleus and position of the generative nucleus in the pollen tube during in vitro germination were examined for six bromeliad cultivars. The influence of mixed amino acids (casein hydrolysate) and individual amino acids (Arg, Asn, Asp, Glu, Gly, Met, Phe, Orn, Tyr) were tested. Aechmea fasciata and A. chantinii pollen tubes showed more generative nuclear division in cultured pollen tubes than the other four cultivars tested. Casein hydrolysate did not stimulate generative nuclear division. In general arginine (1 mM) improved division of the Aechmea generative nucleus and to a lesser extent this of Vriesea `Christiane', Guzmania lingulata and Tillandsia cyanea. A concentration of 2 mM arginine reduced pollen tube growth of Aechmea. The vegetative nucleus was ahead of the generative nucleus in approximately 50% of the pollen tubes of all cultivars studied. In about 25% of the pollen tubes, the generative nucleus was ahead and in ±25% pollen tubes the vegetative and generative nuclei were joined together. The distance between the two generative nuclei and the distance from the generative nuclei to the pollen tube tip differed significantly for Aechmea fasciata and A. chantinii. The influence of different amino acids for Aechmea fasciata and A. chantinii varied with respect to pollen germination and generative nuclear division. Arg and Met improved nuclear division of both Aechmea cultivars. Pollen germination and sperm cell production were not linked. This information is important to ameliorate in vitro pollination methods used to overcome fertilization barriers in Bromeliaceae and other higher plants.
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References
Brewbaker JL (1967) The distribution and phylogenetic significance of binucleate and trinucleate pollen grains in Angiosperms. Am. J. Bot. 54: 1069–1083
Brewbaker JL &; Kwack BH (1963) The essential role of calcium in pollen germination and pollen tube growth. Am. J. Bot. 50: 859–865
Coleman AW &; Goff LJ (1985) Applications of fluorochromes to pollen biology. I: Mithramycin and 4′,6-diamidino-2-phenylin-dole (DAPI) as vital stains for quantitation of nuclear DNA. Stain Technol. 60: 145–154
Cresti M, Ciampolini F &; Sarfatti G (1980) Ultrastructural investigations on Lycopersicon peruvianum pollen activation and pollen tube organization after self-and cross-pollination. Planta 150: 211–217
George EF (1993) Plant Propagation by Tissue Culture. Part 1. The Technology. 2nd edn. (574). Exegetics Lt, London
de Graaf BHJ, Derksen JWM &; Mariani C (2001) Pollen and pistil in the progamic phase. Sex. Plant Reprod. 14: 41–55
Heslop-Harrison J, Heslop-Harrison Y &; Shivanna KR (1984) The evaluation of pollen quality, and a further appraisal of the fluorochromatic (FCR) test procedure. Theor. Appl. Genet. 67: 367–375
Higashiyama T, Kuroiwa H, Kawano S &; Kuroiwa T (1998) Guidance in vitro of the pollen tube to the naked embryo sac of Torenia fournieri. Plant Cell 10: 2019–2031
Jauh GY, Eckard KJ, Nothnagel EA &; Lord EM (1997) Adhesion of lily pollen tubes to an artificial matrix. Sex. Plant Reprod. 10: 173–180
Kakeda K, Jordan ND, Conner A, Ride JP, Franklin-Tong VE &; Franklin FC (1998) Identification of residues in a hydrophilic loop of the Papaver rhoeas S protein that play a crucial role in recognition of incompatible pollen. Plant Cell 10: 1723–1731
Mulcahy DL &; Mulcahy GB (1988) The effect of supplemented media in vitro on bi-and trinucleate pollen. Plant Sci. 55: 213–216
Parton E, Vervaeke I, Delen R, Vandenbussche B, Deroose R &; De Proft MP (2002) Viability and storage of bromeliad pollen. Euphytica 125: 155–161
Raghavan V (1997) Molecular Embryology of Flowering Plants (690). Cambridge University Press, Cambridge
Read SM, Clarke AE &; Bacic A (1993) Requirements for division of the generative nucleus in cultured pollen tubes of Nicotiana. Protoplasma 174: 101–115
Tanaka I (1997) Differentiation of generative and vegetative cells in Angiosperm pollen. Sex. Plant Reprod. 10: 1–7
Tian HQ, Zhang Z &; Russell SD (2001) Sperm dimorphism in Nicotiana tabacum L. Sex. Plant Reprod. 14: 123–125
Vervaeke I, Parton E, Maene L, Deroose R &; De Proft MP (2001) Prefertilization barriers between different Bromeliaceae. Euphytica 118: 91–97
Vervaeke I, Parton E, Deroose R &; De Proft MP (2002a) Control-ling prefertilization barriers by in vitro pollination and fertiliza-tion of Bromeliaceae. Acta Hort. 572: 21–28
Vervaeke I, Parton E, Maene L, Deroose R &; De Proft MP (2002b) Pollen tube growth and fertilization after different in vitro pollination techniques of Aechmea fasciata. Euphytica 124: 75–83
Williams JH, Friedman WE &; Arnold ML (1999) Developmental selection within the angiosperm style: using gamete DNA to visualize interspecific pollen competition. Proc. Natl. Acad. Sci. USA 96: 9201–9206
Zhang G, Liu D &; Cass DD (1997) Calcium-induced sperm fusion in Zea mays L. Sex. Plant Reprod. 10: 74–82
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Vervaeke, I., Delen, R., Wouters, J. et al. Division of the generative nucleus in cultured pollen tubes of the Bromeliaceae. Plant Cell, Tissue and Organ Culture 76, 17–28 (2004). https://doi.org/10.1023/A:1025816722684
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DOI: https://doi.org/10.1023/A:1025816722684