Behavior Genetics

, Volume 27, Issue 2, pp 125–132

A Simple Method to Calculate Resolving Power and Confidence Interval of QTL Map Location

Article

DOI: 10.1023/A:1025685324830

Cite this article as:
Darvasi, A. & Soller, M. Behav Genet (1997) 27: 125. doi:10.1023/A:1025685324830

Abstract

“Resolving power” is defined as the 95% confidence interval for quantitative trait locus (QTL) map location that would be obtained when scoring an infinite number of markers in a given constellation of a marker-QTL mapping experiment. Resolving power can serve as a close estimate of the confidence interval of QTL map location, as well as a guide to the lower efficient limit of marker spacing in an initial marker-QTL mapping experiment. In the present study, an extensive series of simulations was carried out to provide estimates of resolving power, for backcross (BC) and F2 designs, over a wide range of experimental sizes and of gene effects and dominance at the QTL. From the simulation results, the remarkably simple expressions, 3000/(mNd2) (where m = 1 for BC and m = 2 for F2; N = population size, and d = allele substitution effect) and 530/Nν (in terms of ν, the proportion of variance explained), were obtained for estimating resolving power. These expressions can provide a convenient guide to planning marker spacing in BC and F2 marker-QTL linkage experiments and for placing confidence intervals about QTL map location obtained in such experiments.

Quantitative trait loci (QTL)confidence intervalresolving powergene mapping

Copyright information

© Plenum Publishing Corporation 1997

Authors and Affiliations

  1. 1.Department of Genetics, The Alexander Silberman Life Sciences InstituteThe Hebrew University of JerusalemJerusalemIsrael
  2. 2.The Jackson LaboratoryBar Harbor