Molecular and Cellular Biochemistry

, Volume 252, Issue 1, pp 397–400

Resistin expression in different adipose tissue depots during rat development

  • Paula Oliver
  • Catalina Picó
  • Francisca Serra
  • Andreu Palou
Article

DOI: 10.1023/A:1025500605884

Cite this article as:
Oliver, P., Picó, C., Serra, F. et al. Mol Cell Biochem (2003) 252: 397. doi:10.1023/A:1025500605884

Abstract

Resistin is a hormonal factor synthesised by adipocytes that was first thought to be related with the resistance to insulin in obesity, but whose function is not yet completely established. Here we have studied the ontogenic pattern of resistin mRNA expression in different white adipose tissue depots (WAT) – epididymal, inguinal, mesenteric and retroperitoneal – and in brown adipose tissue (BAT), as well as the circulating resistin levels, in rats of different ages (from the suckling period to one year of age). Resistin mRNA was determined by Northern blotting, and serum levels by enzyme immunoassay. In WAT, resistin expression remains almost constant with age, except in early development, where there is a peak of expression in the epididymal and retroperitoneal depots, and a decrease in the inguinal one, while the expression remains constant for the mesenteric depot. Moreover, there is a site-specific difference regarding resistin expression: all the depots express characteristic levels of mRNA, especially at the age of 2 months, the moment when resistin mRNA levels are significantly higher in the epididymal and the retroperitoneal than in the inguinal and mesenteric WAT and than in the BAT. The transient increased resistin expression in the epididymal and the retroperitoneal WAT at a period of time in which there is a change in diet (from milk to chow) suggests a common nutritional regulation of the resistin gene. Circulating resistin levels increase with age probably reflecting the increase in the body fat content.

resistinadipose tissue depotsrat development

Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • Paula Oliver
    • 1
  • Catalina Picó
    • 1
  • Francisca Serra
    • 1
  • Andreu Palou
    • 1
  1. 1.Laboratori de Biologia Molecular, Nutrició i Biotecnologia, Departament de Biologia Fonamental i Ciències de la SalutUniversitat de les Illes BalearsPalma de MallorcaSpain